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Occurrence of transgenic progenies in the harvest of winter oilseed rape variety trials

机译:在冬季油菜品种试验中收获转基因子代

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摘要

In order to determine the portion of transgenic, herbicide resistant progeny occurring in the non-transgenic harvest during the joint testing of genetically modified and conventional varieties of winter oilseed rape (Brassica napus L.), we harvested variety trials at 12 different test sites. Two random samples per test site of the bulked seed material of the non-transgenic varieties were screened for seedlings resistant to glufosinate by a herbicide germination test, followed by a PCR analysis of resistant seedlings. The average rate of resistant seedlings was 0.53 % with a range of 0.08 to 1.16%. The portion of transgenic seeds differed in part considerably between samples of the same seed lot. Possible reasons for this variation are discussed.
机译:为了确定在冬季转基因油菜和传统品种油菜(Brassica napus L.)的联合测试过程中非转基因收获物中发生的转基因,除草剂抗性子代的比例,我们在12个不同的测试地点进行了品种试验。通过除草剂发芽试验,针对非转基因品种的散装种子材料的每个测试位点随机抽取两个样品,以筛选出对草铵膦具有抗性的幼苗,然后对抗性幼苗进行PCR分析。抗性幼苗的平均比率为0.53%,范围为0.08至1.16%。同一种子批次的样品之间,转基因种子的部分差异很大。讨论了这种变化的可能原因。

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