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A high-resolution analyser for the measurement of ammonium in oligotrophic seawater

机译：一种用于测量贫营养海水中铵态氮的高分辨率分析仪

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摘要

In this work, we describe a high-resolution fluoro-metric shipboard analyser and an improved method to determine NH4+ in oligotrophic seawater. The limit of detection is <5 nM, calculated with 95% confidence level using the weighted regression line applied to the standard addition method using real samples prepared with low nutrient seawater from the Atlantic. The results are summarised and cross-compared with spiked artificial seawater (ASW) and spiked Milli-Q water samples. The analyser has a precision of ±1-4% with a high performance over a wide range from 5 nM to 25 nM. The methodology of NH4+ analysis is based on the fluorescent product formed between o-pthaldialdehyde and NH4+ in the presence of sulfite. Due to the high resolution of the developed system, we were able to study in depth the sensitivity of the method to salinity, amines, amino acids and potential interferences from particles/algae. The method was found to be sensitive to salinity variations, reducing the signal by up to 85% at 5 nM; this effect decreased at higher concentrations of ammonium. It was noted that the interference from amines at low concentrations was negligible; however, at either high amino acid or high amine concentrations, the signal was depressed. To test for the effect of particles on the system, the system was tested with samples containing phytoplankton {Dunaliella primolecta) cells at different concentrations prepared with ASW to simulate the effect of a phytoplankton bloom. This experiment assessed the potential impact of both particles and other potential fluorescence interferences from cells and/or ammonium leaching from cells. This experiment showed that a phytoplankton bloom could potentially have an impact of up to 12% on the signal of interest. Thus, we propose that this method is suitable for oligotrophic environments rather than coastal and eutrophic environments. The reagent was found to be stable for 17 days and standards of 1 jxM were stable for 6 days under laboratory conditions. The developed analyser was successfully demonstrated in the North Atlantic Ocean, in an area of oligotrophic, low NH4+ oceanic waters.
机译：在这项工作中，我们描述了一种高分辨率的荧光测量船载分析仪和一种改进的方法来测定贫营养海水中的NH4 +。检出限<5 nM，使用适用于标准添加方法的加权回归线（使用来自大西洋的低营养海水制备的真实样品），以95％置信度计算得出。对结果进行了总结，并与加标的人造海水（ASW）和加标的Milli-Q水样品进行了交叉比较。该分析仪的精度为±1-4％，在5 nM至25 nM的宽范围内均具有高性能。 NH4 +分析的方法基于在亚硫酸盐存在下邻邻苯二甲醛和NH4 +之间形成的荧光产物。由于所开发系统的高分辨率，我们能够深入研究该方法对盐度，胺，氨基酸和颗粒/藻类潜在干扰的敏感性。发现该方法对盐度变化敏感，在5 nM时将信号降低多达85％。在较高的铵浓度下，这种作用减弱。注意到低浓度的胺干扰可忽略不计；但是，在高氨基酸或高胺浓度下，信号均被抑制。为了测试颗粒对系统的影响，用含有不同浓度的浮游植物（Dunaliella primolecta）细胞的样品对系统进行了测试，用ASW制备了这些细胞以模拟浮游植物开花的效果。该实验评估了颗粒的潜在影响以及来自细胞的其他潜在荧光干扰和/或来自细胞的铵浸出。该实验表明，浮游植物的开花可能对目标信号产生多达12％的影响。因此，我们建议该方法适用于贫营养环境而不是沿海和富营养环境。发现该试剂在实验室条件下稳定17天，标准1 jxM稳定6天。研制成功的分析仪已在北大西洋低营养，低NH4 +的海洋水域成功进行了演示。

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  • 来源
    《Ocean Dynamics》 |2011年第10期|p.1555-1565|共11页
  • 作者

    S. K. Abi Kaed Bey; D. P. Connelly; F.-E. Legiret; A. J. K. Harris; M. C. Mowlem;

  • 作者单位

    National Oceanography Centre,University of Southampton—Waterfront Campus,European Way,Southampton, UK SO 14 3ZH;

    National Oceanography Centre,University of Southampton—Waterfront Campus,European Way,Southampton, UK SO 14 3ZH;

    National Oceanography Centre,University of Southampton—Waterfront Campus,European Way,Southampton, UK SO 14 3ZH;

    National Oceanography Centre,University of Southampton—Waterfront Campus,European Way,Southampton, UK SO 14 3ZH;

    National Oceanography Centre,University of Southampton—Waterfront Campus,European Way,Southampton, UK SO 14 3ZH;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    ammonium; shipboard; analyser; fluorometric; resolution;

    机译：铵船上分析器荧光法解析度;

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