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Carbon Chemistry: Carbonate Systems

机译:碳化学:碳酸盐系统

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Bacteria in the Arctic Waters are well adapted to low temperatures and play a key role in the transformation of organic matter. However, the activity of planktonic bacteria at cellular level remains poorly understood. In this study, we use fluorescent markers (4',6'-diamidino-2-phenylindole (DAPI), 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), Live/Dead BacLight viability kit) to discriminate between bacterial cells with a variety of physiological activities in the 0-200 m water column and sinking particles. During two field studies (July 2003 and 2004), we covered nine stations in the northern Barents Sea. The median bacterial abundance (DAPI staining) in the upper 50 m layer was 0.9×10~6 cells ml~(-1) (range 0.2-3.2×10~6 cells ml~(-1)) in 2003 and 0.5×10~6 cells ml1 (range 0.2-1.0×10~6 cells ml1) in 2004. Bacteria with sufficient electron transport activity to be stained with CTC were on average 10% of the total count and ca. 20% of the total cells had intact cell membranes. In the water column, proxies of substrate availability (POC, PON, chlorophyll a, primary production) and bacterial production (thymidine and leucine uptake) correlated strongly with total bacterial count, CTC-stained cells and cells with 'leaky' membrane (stained with propidium iodine), but not with the concentration of cells with intact cell membrane.
机译:北极水域中的细菌非常适合低温,并在有机物转化中起关键作用。但是,关于浮游细菌在细胞水平上的活性仍然知之甚少。在这项研究中,我们使用荧光标记(4',6'-二mid基-2-苯基吲哚(DAPI),5-氰基-2,3-二甲苯基四唑氯化物(CTC),活/死BacLight生存力试剂盒)来区分细菌细胞在0-200 m的水柱中具有多种生理活动并下沉颗粒。在两次现场研究(2003年7月和2004年7月)中,我们覆盖了北巴伦支海的9个站。上部50 m层的细菌中位数(DAPI染色)在2003年为0.9×10〜6细胞ml〜(-1)(范围0.2-3.2×10〜6细胞ml〜(-1))和0.5×10 2004年,约有6个细胞ml1(范围为0.2-1.0×10〜6个细胞ml1)。具有足够的电子传输活性以被CTC染色的细菌平均占总数的10%,大约为1。总细胞中有20%具有完整的细胞膜。在水柱中,底物有效性(POC,PON,叶绿素a,初级产量)和细菌产量(胸苷和亮氨酸吸收)的代理与细菌总数,CTC染色的细胞和带有“渗漏”膜的细胞(染色的)密切相关。碘化丙啶),但不与完整细胞膜的细胞浓度有关。

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    《Oceanographic Literature Review》 |2009年第3期|540-542|共3页
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