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首页> 外文期刊>Optical and quantum electronics >In vitro human serum albumin glycation monitored by Terahertz spectroscopy
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In vitro human serum albumin glycation monitored by Terahertz spectroscopy

机译：太赫兹光谱法监测体外人血清白蛋白糖基化

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摘要

The nonenzymatic attachment of sugars to proteins, namely glycation, is accelerated under diabetic conditions. Monitoring the glycated human serum albumin (HSA) levels gives the short term variation of glucose concentration in diabetic patients blood. Therefore, a significant effort was made to measure glycated HSA, including by spec-troscopic methods such as Raman. Here we used THz spectroscopy to monitor HSA glycation in time (over 5, 7 and 11 weeks). Different sugar types have different reactivity; therefore we also addressed the reducing sugar influence on glycation by performing in vitro HSA glycation by both glucose and fructose. Since residues protonation state influences their susceptibility for glycation, we incubated HSA with sugars at two pH values: 7 and 8. Our results show that THz absorption decreases with the incubation time of HSA with sugars. At the incubation times we considered, the most significant differences were obtained on HSA samples glycated using glucose. Differences between samples glycated by glucose and by fructose show that glycation by glucose is a slower process. At pH 7, glycation by glucose is slower than at pH 8, while glycation by fructose is slightly faster at pH 7 than at pH 8. Glycated HSA models with different degrees of glycation were built by molecular modeling. Simulated THz spectra of the models are in good agreement with the experimental data. All these show that THz spectroscopy could monitor the progression of glycation in time and that it is sensitive to reducing sugars or pH value used in the glycation process.
机译：在糖尿病条件下，糖与蛋白质的非酶连接（即糖基化）被加速。监测糖化的人血清白蛋白（HSA）水平可得出糖尿病患者血液中葡萄糖浓度的短期变化。因此，付出了巨大的努力来测量糖化的HSA，包括通过诸如拉曼光谱法的方法。在这里，我们使用太赫兹光谱来及时（超过5、7和11周）监测HSA糖化。不同的糖类型具有不同的反应性。因此，我们还通过葡萄糖和果糖进行体外HSA糖基化处理来解决还原糖对糖基化的影响。由于残基的质子化状态会影响其糖基化敏感性，因此我们将HSA与糖在两个pH值（7和8）下孵育。我们的结果表明，随着HSA与糖孵育时间的延长，THz吸收降低。在我们考虑的孵育时间中，使用葡萄糖糖化的HSA样品获得了最显着的差异。葡萄糖和果糖糖基化的样品之间的差异表明，葡萄糖糖基化是一个较慢的过程。在pH 7时，葡萄糖的糖基化速度比在pH 8时慢，而在pH 7时果糖的糖化速度稍快于在pH 8时。通过分子建模建立了糖化程度不同的糖化HSA模型。模型的模拟太赫兹光谱与实验数据吻合良好。所有这些表明，太赫兹光谱法可以及时监测糖基化的进程，并且对糖基化过程中使用的还原糖或pH值敏感。

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  • 来源
    《Optical and quantum electronics》 |2015年第4期|961-973|共13页
  • 作者

    Maria Mernea; Alina Ionescu; Ionut Vasile; Cristina Nica; Gheorghe Stoian; Traian Dascalu; Dan Florin Mihailescu;

  • 作者单位

    Faculty of Biology, University of Bucharest, Splaiul Independentei, No 91-95, 050095 Bucharest, Romania;

    Faculty of Biology, University of Bucharest, Splaiul Independentei, No 91-95, 050095 Bucharest, Romania,Laboratory of Solid-State Quantum Electronics, National Institute for Laser, Plasma and Radiation Physics, PO Box MG-36, 077125 Magurele, Romania;

    Doctoral School of Physics, University of Bucharest, Atomistilor Street, No 405, 077125 Magurele, Romania,Horia Hulubei National Institute for Physics and Nuclear Engineering, PO Box MG-6, 077125 Magurele, Romania;

    Faculty of Biology, University of Bucharest, Splaiul Independentei, No 91-95, 050095 Bucharest, Romania;

    Faculty of Biology, University of Bucharest, Splaiul Independentei, No 91-95, 050095 Bucharest, Romania;

    Laboratory of Solid-State Quantum Electronics, National Institute for Laser, Plasma and Radiation Physics, PO Box MG-36, 077125 Magurele, Romania;

    Faculty of Biology, University of Bucharest, Splaiul Independentei, No 91-95, 050095 Bucharest, Romania;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    THz time-domain attenuated total reflection spectroscopy; Molecular modeling; Protein glycation; Advanced glycation end-products;

    机译：太赫兹时域衰减全反射光谱;分子建模;蛋白质糖基化;高级糖化终产物;

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