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Achromatization method for multichannel fluorescence imaging systems

机译：多通道荧光成像系统的消色差方法

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摘要

An achromatization method optimized for dual-channel imaging is developed. Dichroic mirrors are employed to split and recombine narrowband signals, and separation between catoptric components is used to minimize the longitudinal chromatic shift. An achromatic system based on this principle could be built from singlet lenses, since refractive element properties such as dispersion and power are not utilized to optimize wavelength-dependent performance. To demonstrate the validity of the proposed solution, a prototype miniature fluorescence microscope optimized for two emission lines of acridine orange (525 and 650 nm) is built. To reduce the cost and accelerate assembly, the system is built from commercially available optical components. The optical train consisted of two plastic singlet lenses combined with a pair of dichroic mirrors. Optical performance of the prototype is evaluated by imaging a bar line target at both design wavelengths. To demonstrate the potential of the proposed design strategy, the achromatic system prototype is used to measure a two-part white blood cells differential count on a venous blood sample. Data from the prototype fluorescence microscope are compared against results from a commercially available blood analyzer, and the difference between both instruments is Within 20%.
机译：开发了一种针对双通道成像优化的消色差方法。二向色镜用于分离和重组窄带信号，并且反射分量之间的分隔用于最小化纵向色移。基于该原理的消色差系统可以由单重透镜构成，因为不利用诸如色散和屈光度之类的折射元件特性来优化与波长有关的性能。为了证明所提出解决方案的有效性，建立了一个原型微型荧光显微镜，该显微镜针对for啶橙的两条发射线（525和650 nm）进行了优化。为了降低成本并加快组装速度，该系统由市售的光学组件组成。光学系统由两个塑料单重透镜和一对二向色镜组成。原型的光学性能通过在两个设计波长下成像条状靶标来评估。为了证明所提出的设计策略的潜力，消色差系统原型用于测量静脉血样上两部分的白细胞差异计数。将原型荧光显微镜的数据与市售血液分析仪的结果进行比较，两种仪器之间的差异在20％之内。

著录项

  • 来源
    《Optical engineering》 |2019年第1期|015106.1-015106.9|共9页
  • 作者

    Michal E. Pawlowski; Yiran Yang; Cynthia Wong; Tomasz S. Tkaczyk;

  • 作者单位

    Rice University, Department of Bioengineering, Houston, Texas, United States;

    Rice University, Department of Bioengineering, Houston, Texas, United States,California Institute of Technology, Department of Medical Engineering, Pasadena, California, United States;

    Rice University, Department of Bioengineering, Houston, Texas, United States;

    Rice University, Department of Bioengineering, Houston, Texas, United States,Rice University, Department of Electrical and Computer Engineering, Houston, Texas, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    fluorescence microscopy; optical design and fabrication; medical optics and biotechnology; diagnostic instrumentation;

    机译：荧光显微镜光学设计和制造;医疗光学和生物技术;诊断仪器;

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