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Colony image acquisition system and segmentation algorithms

机译:菌落图像采集系统及分割算法

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摘要

This paper presents a novel colony analysis system in-cluding an adjustable image acquisition subsystem and a wavelet-watershed-based image segmentation algorithm. An illumination box was constructed—both front lightning and back lightning illuminations can be chosen by users based on the properties of Petri dishes. In the illumina-tion box, the lightning is uniform, which makes image processing easy. A digital camera at the top of the box is connected to a PC computer; all the camera functions are controlled by the developed computer soft-ware in this study. As usual, in the image processing part, the hardest task is image segmentation which is carried out by the four different al-gorithms: 1. recursive image segmentation on gray similarity; 2. canny edge detection-based segmentation; 3. the combination of 1 and 2, and 4. colony delineation on wavelet and watershed. The first three algorithms can obtain good results for ordinary colony images, and for the images in-cluding a lot of small (tiny) colonies and dark colonies and overlapping (or touching) colonies, the algorithm 4 can obtain better results than the oth-ers. The algorithms are tested by using a large number of different colony images, and the testing results are satisfactory.
机译:本文提出了一种新颖的菌落分析系统,其中包括一个可调节的图像采集子系统和一个基于小波分水岭的图像分割算法。构造了一个照明箱-用户可以根据培养皿的特性选择前闪电照明和后闪电照明。在照明箱中,闪电均匀,这使得图像处理变得容易。盒子顶部的数码相机连接到PC计算机。在这项研究中,所有相机功能均由开发的计算机软件控制。像往常一样,在图像处理部分,最困难的任务是通过四个不同的算法进行图像分割:1.基于灰度相似度的递归图像分割; 2.基于Canny边缘检测的分割; 3. 1和2的组合,以及4.小波和分水岭上的菌落轮廓。前三种算法对于普通菌落图像可以获得良好的结果,对于包括很多小(微小)菌落,暗菌落和重叠(或接触)菌落的图像,算法4可以获得比其他菌落更好的结果。呃。通过使用大量不同的菌落图像对算法进行测试,测试结果令人满意。

著录项

  • 来源
    《Optical engineering》 |2011年第12期|p.123001.1-123001.9|共9页
  • 作者

    Weixing Wang;

  • 作者单位

    Chang'an University School of Information Engineering 710064 Xi'an, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    colony image; segmentation; wavelet; image acquisition;

    机译:殖民地形象分割;小波图像采集;

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