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A highly selective LSPR biosensor for the detection of taurine realized on optical fiber substrate and gold nanoparticles

机译：在光纤基质和金纳米颗粒上实现的用于牛磺酸检测的高选择性LSPR生物传感器

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摘要

In the current study, we report an enzyme based fiber optic biosensor utilizing the localized surface plasmon resonance (LSPR) of gold nanoparticles for the detection of taurine in the concentration range of 0-1 mM. For probe fabrication, gold nano-particles are decorated on an unclad portion of multimode optical fiber, and taurine dioxygenase enzyme is immobilized over them. The presence of taurine and its co-reactants alpha-ketoglutarate and oxygen in the vicinity of the probe activates the immobilized enzyme which elicits the chemical reaction that gets manifested in the form of refractive index change in the surrounding of gold nanoparticles. This changes the absorbance spectrum of the nanoparticles recorded at the output end of the fiber probe. The absorbance spectrum changes in terms of absorbance and peak absorbance wavelength. A change of 0.0115 in absorbance value is obtained for the change in taurine concentration from 0 to 1 mM. The limit of detection and the sensitivity of the sensor are found to be 53 mu M and 0.0190 AU/mM, respectively. The sensor response is found to be stable and selective along with good repeatability. Being fabricated on optical fiber substrate, the sensor possesses the inherent advantages like online and remote monitoring of taurine along with immunity to electromagnetic noise. Moreover, the reported sensor has simple operation and is low in cost.
机译：在当前的研究中，我们报告了一种基于酶的光纤生物传感器，该传感器利用金纳米颗粒的局部表面等离振子共振（LSPR）检测浓度为0-1 mM的牛磺酸。对于探针的制造，将金纳米颗粒装饰在多模光纤的未覆盖部分上，并将牛磺酸双加氧酶固定在其上。牛磺酸及其共反应物α-酮戊二酸和氧在探针附近的存在会激活固定化酶，从而引发化学反应，该化学反应以金纳米颗粒周围折射率变化的形式表现出来。这改变了记录在纤维探针输出端的纳米颗粒的吸收光谱。吸收光谱根据吸收和峰值吸收波长而变化。对于牛磺酸浓度从0到1 mM的变化，吸光度值的变化为0.0115。检出限和传感器的灵敏度分别为53μM和0.0190 AU / mM。发现传感器响应是稳定且选择性的以及良好的可重复性。该传感器在光纤基板上制造，具有固有的优势，例如在线和远程监测牛磺酸以及对电磁噪声的免疫力。此外，所报道的传感器具有简单的操作并且成本低。

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