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首页> 外文期刊>Optical Materials >Gold nanoparticle-mediated fluorescence enhancement by two-photon polymerized 3D microstructures
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Gold nanoparticle-mediated fluorescence enhancement by two-photon polymerized 3D microstructures

机译：金纳米粒子介导的双光子聚合3D微观结构的荧光增强

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摘要

Fluorescence enhancement achieved by functionalized microstructures made by two-photon polymerization (TPP) is reported for the first time. Microstructures of various shapes made of SU-8 photoresist were prepared and coated with gold nanoparticles (NP) of 80 nm. Localized fluorescence enhancement was demonstrated by microstructures equipped with tips of sub-micron dimensions. The enhancement was realized by positioning the NP-coated structures over fluorescent protein layers. Two fluorophores with their absorption in the red and in the green region of the VIS spectrum were used. Laser scanning confocal microscopy was used to quantify the enhancement. The enhancement factor was as high as 6 in areas of several square-micrometers and more than 3 in the case of local enhancement, comparable with literature values for similar nanoparticles. The structured pattern of the observed fluorescence intensity indicates a classic enhancement mechanism realized by standing waves over reflecting surfaces. With further development mobile microtools made by TPP and functionalized by metal NPs can be actuated by optical tweezers and position to any fluorescent micro-object, such as single cells to realize localized, targeted fluorescence enhancement.
机译：首次报道了通过双光子聚合（TPP）制成的功能化微结构实现的荧光增强。制备了由SU-8光刻胶制成的各种形状的微结构，并用80 nm的金纳米颗粒（NP）涂覆。通过配备亚微米尺寸尖端的微结构证明了局部荧光增强。通过在荧光蛋白层上放置NP涂层结构来实现增强。使用了两种在VIS光谱的红色和绿色区域吸收的荧光团。使用激光扫描共聚焦显微镜定量增强。在几平方微米的区域中，增强因子高达6，而在局部增强的情况下，增强因子则大于3，与相似的纳米粒子的文献值相当。观察到的荧光强度的结构化模式表明了经典的增强机制，该机制是通过在反射表面上驻波实现的。随着进一步的发展，由TPP制造并由金属NPs功能化的移动微型工具可以通过光镊启动，并定位到任何荧光微物体（例如单个细胞）上，以实现定位的，有针对性的荧光增强。

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