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Poly-tryptophan/carbazole based FRET-system for sensitive detection of nitroaromatic explosives

机译：基于聚色氨酸/咔唑的FRET系统用于硝基芳香族炸药的灵敏检测

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摘要

Sensitive detection of nitroaromatic explosives, a hot topic of worldwide concern, is greatly asked for, due to the adverse effect on ecosystems and human health originating from the excessive use and inadequate management. Here, we report the development of a Forster Resonance Energy Transfer (FRET) based sensor system for highly sensitive detection of nitro-aromatic compounds (NACs). The sensor system is composed of a copolymer poly[(N, N-dimethylacrylamide)-co-(Boc-Trp-EMA)] (RP) bearing tryptophan derivative in the side chain as donor and carbazole (CZ) as an acceptor. Primarily, the fluorescence of RP copolymer is quenched by non-radiative energy transfer to CZ which only happens once the two molecules are within Forster critical distance. The efficiency and rate of energy transfer process were calculated for this deliberated FRET system. This FRET pair exhibited a specific fluorescence response to NACs such as 2,4,6-trinitrophenol (TNP), 2,4-dinitrotoluene (DNT) and 2,4,6-trinitrotoluene (TNT) with 6.4, 5.9 and 1.4 mu M limit of detections (LODs), respectively. Interestingly, the order of sensitivities of the FRET pair to NACs was very high. The sensing process occurs through a static and dynamic quenching pathway, confirmed by Stern-Volmer analysis of steady-state and time-resolved fluorescence as well as UV-Vis Spectroscopy.
机译：由于过度使用和管理不当对生态系统和人类健康造成不利影响，因此强烈要求敏感检测硝基芳族炸药，这是全世界关注的热点。在这里，我们报告基于Forster共振能量转移（FRET）的传感器系统的发展，该系统可用于高灵敏度地检测硝基芳族化合物（NAC）。传感器系统由在侧链带有色氨酸衍生物作为供体和咔唑（CZ）作为受体的聚[（N，N-二甲基丙烯酰胺）-共-（Boc-Trp-EMA）]共（RP）共聚物组成。首先，RP共聚物的荧光通过向CZ的非辐射能量转移而淬灭，只有当两个分子都在Forster临界距离内时才会发生。计算出了针对该FRET系统的效率和能量传递过程的速率。该FRET对在6.4、5.9和1.4μM的条件下对NAC（例如2,4,6-三硝基苯酚（TNP），2,4-二硝基甲苯（DNT）和2,4,6-三硝基甲苯（TNT））表现出特定的荧光响应检出限（LOD）。有趣的是，FRET对对NAC的敏感性很高。传感过程是通过静态和动态猝灭途径发生的，通过稳态和时间分辨荧光的Stern-Volmer分析以及​​UV-Vis光谱学证实了这一过程。

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