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Wavelength-coded multifocal microscopy

机译:波长编码多焦点显微镜

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摘要

A wavelength-coded multifocal microscope incorporating multiplexed and wavelength-coded holographic gratings to generate wavelength-selective multifocal planes is presented. The focal planes are longitudinally spaced on the object plane, and each focal plane is probed by a designated wavelength. The recording of the multiplexed gratings takes place at a single wavelength by utilizing the Bragg degeneracy property; thus the maximum sensitive wavelength of blue 488   nm is used for recording, but the device is operated at a broad wavelength band of interest, all the way to red 633   nm . We present the design, implementation, and experimental image data demonstrating this microscope’s ability to obtain biological tissue structures simultaneously at different focal planes using broadband illumination by LEDs.
机译:提出了一种波长编码的多焦点显微镜,该显微镜结合了复用的和波长编码的全息光栅以产生波长选择的多焦点平面。焦平面在物平面上纵向隔开,并且每个焦平面由指定的波长探测。利用Bragg简并特性,可以在单个波长下记录多路复用光栅。因此,使用蓝色488 nm的最大敏感波长进行记录,但是该设备在感兴趣的较宽波段内操作,一直到红色633 nm。我们介绍了设计,实现和实验图像数据,这些数据证明了该显微镜具有使用LED的宽带照明在不同焦平面上同时获取生物组织结构的能力。

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  • 来源
    《Optics Letters》 |2010年第5期|p.781-783|共3页
  • 作者单位

    1Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA 2Singapore-MIT Alliance for Research and Technology (SMART) Centre, Massachusetts Institute of Technology, S16-06-17, 3 Science Drive 2, Singapore 117543;

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