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Investigation of antibacterial activity of polyvinyl alcohol packaging films composed of silver oxide nanoparticles, graphene oxide and tragacanth gum using Box-Behnken design

机译:用盒-Behnken设计研究由氧化银纳米粒子,石墨烯和Tragacanth Gum组成的聚乙烯醇包装膜的抗菌活性研究

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摘要

The present study aimed to develop an antibacterial packaging system based on biodegradable polyvinyl alcohol (PVA) polymer. Box-Behnken design was used to investigate silver oxide nanoparticles, graphene oxide and tragacanth gum as film additives to obtain maximum antibacterial activity against Staphylococcus aureus and Escherichia coli. The experimental design showed the inhibitory effect of graphene oxide and tragacanth gum on the antibacterial activity of PVA films containing Ag2O nanoparticles. The optimum film containing 5% Ag2O nanoparticles (% of PVA weight) was prepared and characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric/differential thermal analyser (TG-DTA). Results of this study showed the formation of PVA film with 0.04-mm thickness, with a higher density of Ag2O nanoparticles (25- to 75-nm size) around the upper surface. Compared with blank PVA film, the optimum film showed higher tensile strength and thermal stability. It was also more efficient in water vapour and oxygen barrier properties, along with preserving raw beef samples by inhibiting the growth of beef natural bacteria for at least 7 days, when stored in the refrigerator.
机译:本研究旨在发展基于可生物降解的聚乙烯醇(PVA)聚合物的抗菌包装系统。 Box-Behnken设计用于研究氧化银纳米颗粒,石墨烯和Tragacanth Gum作为膜添加剂,以获得对金黄色葡萄球菌和大肠杆菌的最大抗菌活性。实验设计表明,石墨烯和曲折胶对含有Ag2O纳米颗粒的PVA膜的抗菌活性的抑制作用。使用扫描电子显微镜和能量分散X射线光谱(SEM-EDS),傅里叶变换红外光谱(FTIR)和热重分析/差分热分析仪制备含有5%Ag2O纳米颗粒(占PVA重量%的PVA重量%)的最佳薄膜并表征TG-DTA)。该研究的结果表明,厚度为0.04mm厚的PVA膜,围绕上表面呈较高的Ag 2 O纳米颗粒(25-至75〜75mm)的密度。与空白PVA薄膜相比,最佳薄膜显示出更高的拉伸强度和热稳定性。当储存在冰箱中时,在水蒸气和氧气阻隔性中,通过抑制牛肉天然细菌的生长至少7天,在水蒸气和氧气阻隔性中更有效。

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