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Mitochondrial Responses of Normal and Injured Human Skin Fibroblasts Following Low Level Laser Irradiation-An In Vitro Study

机译：低水平激光照射后正常和受伤的人皮肤成纤维细胞的线粒体反应-一项体外研究

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Laser irradiation has proved to be very efficient in speeding and improving the quality of healing in pathological conditions of diverse etiologies. However, the mechanisms by which the beneficial effects are attained are not clear. Mitochondria are the primary phototargets during irradiation. The study aimed to establish if laser irradiation had an effect on hypoxic and acidotic cells. The study also aimed to use existing information regarding the possible mechanism of action (established in wounded cells) and apply these principles to acidic and hypoxic irradiated cells to determine whether laser has a stimulatory or inhibitory effect. Cell cultures were modified to simulate conditions of hypoxia (hypoxic gas mixture 95% N^sub 2^ and 5% O2) and acidosis (pH 6.7) whereas the central scratch model was used to simulate a wound. Cells were irradiated with a helium-neon (632.8 nm, 3 mW cm^sup -2^) laser using 5 or 16 J cm^sup -2^ on days 1 and 4. Mitochondrial responses were measured 1 or 24 h after laser irradiation by assessing changes in mitochondrial membrane potential (MMP), cyclic AMP, intracellular Ca^sup 2+^ and adenosine triphosphate (ATP) cell viability. Hypoxia and acidosis significantly reduced MMP when compared with normal nonirradiated control cells. Wounded, hypoxic and acidotic cells irradiated with 5 J cm^sup -2^ showed an increase in mitochondrial responses when compared with nonirradiated cells while 16 J cm^sup -2^ showed a significant decrease. The study confirmed that laser irradiation with 5 J cm^sup -2^ stimulated an increase in intracellular Ca^sup 2+^ which resulted in an increase in MMP, ATP and cAMP, which ultimately results in photobiomodulation to restore homeostasis of injured cells. [PUBLICATION ABSTRACT]
机译：事实证明，在各种病因的病理学条件下，激光辐照非常有效地加快和改善愈合质量。但是，获得有益效果的机制尚不清楚。线粒体是照射过程中的主要光靶。该研究旨在确定激光照射是否对缺氧和酸中毒细胞产生影响。该研究还旨在利用有关可能的作用机制（在受伤细胞中建立）的现有信息，并将这些原理应用于酸性和低氧照射的细胞，以确定激光是否具有刺激作用或抑制作用。修改细胞培养物以模拟缺氧（缺氧混合气体95％N 2和5 O 2）和酸中毒（pH 6.7）的条件，而中央刮擦模型用于模拟伤口。在第1天和第4天，用5或16 J cm ^ sup -2 ^的氦氖（632.8 nm，3 mW cm ^ sup -2 ^）激光照射细胞。在激光照射后1或24 h测量线粒体反应通过评估线粒体膜电位（MMP），环状AMP，细胞内Ca 2+和三磷酸腺苷（ATP）细胞活力的变化。与正常的未辐照对照细胞相比，低氧和酸中毒可显着降低MMP。与未照射的细胞相比，用5 J cm ^ sup -2 ^照射的受伤，缺氧和酸中毒细胞显示线粒体反应增加，而16 J cm ^ sup -2 ^则显示显着降低。研究证实用5 J cm ^ sup -2 ^进行的激光照射刺激了细胞内Ca ^ sup 2 + ^的增加，从而导致MMP，ATP和cAMP的增加，最终导致了光生物调节以恢复受损细胞的体内平衡。 [出版物摘要]

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    《Photochemistry and Photobiology》 |2009年第4期|p.987-996|共10页
  • 作者

    Innocent L Zungu Denise Hawkins Evans Heidi Abrahamse;

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    Innocent L. Zungu, Denise Hawkins Evans and Heidi Abrahamse*Laser Research Group, Faculty of Health Sciences, University of Johannesburg, Johannesburg, South AfricaReceived 15 July 2008, accepted 17 November 2008, DOI: 10.1111/j.1751-1097.2008.00523.x* Corresponding author email: habrahamse@uj.ac.za (Heidi Abrahamse)© 2009 The Authors. Journal Compilation. The American Society of Photobiology 0031-8655/09;

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