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Photoelimination of Streptococcus mutans with two methods of photodynamic and photothermal therapy

机译:用光动力和光热疗法两种方法对变形链球菌进行光消除

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Background: Increasing resistance of oral pathogens to conventional antibacterial agents has resulted to find alternative therapies to overcome resistance development problems; hence this in vitro study was carried out to investigate the efficacy of photoelimination of Streptococcus mutans with two methods of photodynamic and photothermal therapy. Methods: Standard Suspensions of S. mutans were treated in two groups of photodynamic therapy with Toluidine blue O and Rhadachlorin~® and photothermal therapy by EmunDo~® and their individual light sources, then Bacterial suspension from each treatment was subcultured on the surface of Mueller-Hinton agar plates and bacterial growth was assessed. The results were analyzed by analysis of variance and Tukey test (p < 0.05). Results: After treatments significant reduction of S. mutans viability in planktonic culture was observed in both groups of photodynamic and photothermal therapy with no priority. Conclusion: Photoelimination can be a novel modality in the eradication of S. mutans colonies in near future.
机译:背景:口腔病原体对常规抗菌剂的耐药性增强,导致寻找替代疗法来克服耐药性发展问题。因此,进行了这项体外研究,以探讨光动力学和光热疗法两种方法对变形链球菌进行光消除的功效。方法:将变形链球菌的标准悬浮液分为两组,分别采用甲苯胺蓝O和Rhadachlorin〜®进行光动力治疗,并使用EmunDo〜®及其各自的光源进行光热疗法,然后将每种处理的细菌悬浮液在Mueller表面传代培养。 -评估欣顿琼脂平板和细菌生长。通过方差分析和Tukey检验分析结果(p <0.05)。结果:治疗后,在光动力疗法和光热疗法两组中均观察到变形链球菌在浮游培养中的活力显着降低,没有优先考虑。结论:光消除可能是在不久的将来消除变形链球菌菌落的一种新方式。

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