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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Discovering the mechanisms of strain-dependent response of Staphylococcus aureus to photoinactivation: Oxidative stress toleration, endogenous porphyrin level and strain's virulence
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Discovering the mechanisms of strain-dependent response of Staphylococcus aureus to photoinactivation: Oxidative stress toleration, endogenous porphyrin level and strain's virulence

机译：发现金黄色葡萄球菌对光灭活的应变依赖性反应机制：耐氧化应激，内源性卟啉水平和菌株的毒力

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摘要

Background: Staphylococcus aureus is generally known to be susceptible to photoinactivation. However, the phenomenon of its strain-dependent response to photodynamic treatment has been reported. Moreover, the factors determining the emerging variation among strains according to photoinactivation remain unclear. Methods: This work aimed to investigate any relevant correlation between bacterial toleration of oxidative stress, porphyrin level, photosensitizer uptake and strain's virulence of studied methicillin-susceptible and methicillin-resistant S. aureus strains and their response to photo-dynamic inactivation (using protoporphyrin diarginate, toluidine blue 0 and 5-aminolevulinic acid). Results: Obtained data let to demonstrate that studied factors have limited impact on strain response to PDI. However, we have shown that multicomponent sensitizing agent i.e. consisted of PPArg_2, ALA and TBO would eliminate the S. aureus elevated resistance to photoinactivation and that both highly virulent and low virulent S. aureus strains could be easily eradicated with the use of PDI. Moreover, we have shown that photodynamic inactivation could decrease the virulence of S. aureus extracellular fraction. Conclusion: The mechanism underlying strain-dependent response to photoinactivation is complex and multifactorial nevertheless with the use of several sensitizing agents the elevated resistance to photodynamic treatment can be omitted.
机译：背景：众所周知，金黄色葡萄球菌容易发生光灭活。然而，已经报道了其对光动力处理的应变依赖性反应的现象。此外，尚不清楚根据光灭活来确定菌株间新出现变异的因素。方法：这项工作旨在研究所研究的对甲氧西林敏感和耐甲氧西林的金黄色葡萄球菌菌株的细菌对氧化应激的耐受性，卟啉水平，光敏剂吸收和菌株毒力与它们对光动力失活的反应之间的任何相关关系（使用原卟啉二精氨酸） ，甲苯胺蓝0和5-氨基乙酰丙酸）。结果：获得的数据可以证明研究因素对菌株对PDI的响应影响有限。然而，我们已经表明，由PPArg_2，ALA和TBO组成的多组分敏化剂将消除金黄色葡萄球菌对光灭活的提高的抗性，并且使用PDI可以容易地消除高毒性和低毒性金黄色葡萄球菌菌株。此外，我们已经表明，光动力失活可以降低金黄色葡萄球菌细胞外部分的毒力。结论：应变依赖的对光灭活反应的机制很复杂，而且是多因素的，但是通过使用几种敏化剂，可以省去对光动力治疗的抗性。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2013年第4期|348-355|共8页
  • 作者

    Monika Kossakowska; Joanna Nakonieczna; Anna Kawiak; Julianna Kurlenda; Krzysztof P. Bielawski; Mariusz Grinholc PhD;

  • 作者单位

    Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty ofBiotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 Gdansk, Poland;

    Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty ofBiotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 Gdansk, Poland;

    Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty ofBiotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 Gdansk, Poland,Laboratory of Human Physiology, Medical University of Gdansk, Tuwima 15, 80-210 Gdansk, Poland;

    Department of Clinical Bacteriology at the Provincial Hospital, Monte Cassino 13, 75-340 Koszalin, Poland;

    Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty ofBiotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 Gdansk, Poland;

    Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty ofBiotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 Gdansk, Poland;

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  • 正文语种 eng
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  • 关键词

    Endogenous porphyrins; Oxidative stress; Porphyrins; Photoinactivation; S. aureus virulence; Toluidine blue O;

    机译：内源性卟啉;氧化应激卟啉;光灭活;金黄色葡萄球菌毒力;甲苯胺蓝O;

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