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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >In vitro photodynamic effect of aluminum tetrasulfophthalocyanines on melanoma skin cancer and healthy normal skin cells
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In vitro photodynamic effect of aluminum tetrasulfophthalocyanines on melanoma skin cancer and healthy normal skin cells

机译：四硫代酞菁铝对黑素瘤皮肤癌和健康正常皮肤细胞的体外光动力作用

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Photodynamic therapy is a medical treatment that uses an inactive dye/drug and lasers as a light source to activate the dye/drug to produce a toxic form of oxygen that destroys the cancer cells. This study aimed at investigating the cytotoxic effects of different concentrations of aluminum tetrasulfophthalocyanines in its inactive and active state (laser induced) on melanoma skin cancer cells, healthy normal skin fibroblast and keratinocyte cells. Experimentally, 3 × 10~4 cells/ml were seeded in 24-well plates before treatment with different concentrations of aluminum tetrasulfophthalocyanines. After 2h, cells were irradiated with a light dose of 4.5 J/cm~2. Post-irradiated cells were incubated for 24h before cell viability was measured using the CellTiter-Blue Viability Assay. Results showed that aluminum tetrasulfophthalocyanines at high concentrations were cytotoxic to melanoma cells in the absence of laser activation. In the presence of laser activation of aluminum tetrasulfophthalocyanines at a concentration of 40 n,g/ml decreased cell viability of melanoma cells to 45%, flbroblasts to 78% and keratinocytes to 73%. At this photosensitizing concentration of aluminum tetrasulfophthalocyanines the efficacy of the treatment light dose 4.5 J/cm~2 and the cell death mechanism induced by photoactivated aluminum tetrasulfophthalocyanines was evaluated. A light dose of 4.5 J/cm~2 was more efficient in killing a higher number of melanoma cells and a lower number of fibroblast and keratinocyte cells than the other light doses of 2.5 J/cm~2, 7.5 J/cm~2 and 10.5 J/cm~2. Apoptosis features such as blebbing, nucleus condensation, nucleus fragmentation and the formation of apoptotic bodies were seen in the photodynamic therapy treated melanoma skin cancer cells. This in vitro photodynamic therapy study concludes that using aluminum tetrasulfophthalocyanines at a photosensitizing concentration of 40μg/ml in combination with a laser dose of 4.5 J/cm~2 was potentially lethal for melanoma skin cancer cells and less harmful for the normal healthy skin cells.
机译：光动力疗法是一种医学疗法，它使用惰性染料/药物和激光作为光源来激活染料/药物以产生有毒形式的氧气，从而破坏癌细胞。这项研究旨在研究不同浓度的非活性和活性状态（激光诱导）的不同浓度的四硫代酞菁铝对黑素瘤皮肤癌细胞，健康的正常皮肤成纤维细胞和角质形成细胞的细胞毒性作用。实验上，在用不同浓度的四磺基酞菁铝处理之前，将3×10〜4细胞/ ml接种到24孔板中。 2小时后，以4.5J / cm 2的光剂量照射细胞。照射后的细胞孵育24小时，然后使用CellTiter-Blue Viability Assay测定细胞活力。结果表明，在没有激光激活的情况下，高浓度的四硫代酞菁铝对黑素瘤细胞具有细胞毒性。在浓度为40 n，g / ml的四硫代酞菁铝激光激活的情况下，黑素瘤细胞的细胞活力降至45％，成纤维细胞降至78％，角质形成细胞降至73％。在该光敏浓度的四硫代酞菁铝中，评价了处理光剂量为4.5J / cm〜2的功效以及由光活化的四硫代酞菁铝诱导的细胞死亡机制。与其他2.5 J / cm〜2、7.5 J / cm〜2的光剂量相比，4.5 J / cm〜2的光剂量更有效地杀死更多数量的黑素瘤细胞以及更少的成纤维细胞和角质形成细胞。 10.5焦耳/厘米〜2。在光动力疗法治疗的黑色素瘤皮肤癌细胞中观察到凋亡特征，如起泡，核浓缩，核碎裂和凋亡小体的形成。这项体外光动力疗法研究得出的结论是，将光敏浓度为40μg/ ml的四硫代酞菁铝与4.5 J / cm〜2的激光剂量结合使用可能对黑色素瘤皮肤癌细胞具有致命性，而对正常健康皮肤细胞的危害则较小。

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