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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Localization and phototoxic effect of zinc sulfophthalocyanine photosensitizer in human colon (DLD-1) and lung (A549) carcinoma cells (in vitro)
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Localization and phototoxic effect of zinc sulfophthalocyanine photosensitizer in human colon (DLD-1) and lung (A549) carcinoma cells (in vitro)

机译：磺基酞菁锌光敏剂在人结肠（DLD-1）和肺癌（A549）癌细胞中的定位和光毒性作用（体外）

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Background: Photodynamic therapy (PDT) is a therapeutic modality used for treating cancerous cells. It has been previously shown that mixed sulfonated metallophthalocyanine complex, zinc sulfophthalocyanine (ZnPcS_(mix)) is effective in destroying lung cancer cells. This study aimed to determine subcellular localization of ZnPcS_(mix) and its effect on two cancer cell lines. Methods: ZnPcS_(mix) was activated at a wavelength of 680 nm with 5 J/cm~2. Colon (DLD-1) and lung (A549) cancer cell lines were used. Subcellular localization of ZnPcS_(mix) was determined by fluorescence microscopy. Toxicity of PS alone and combination of light and PS (PDT) was determined by cell morphology, viability, proliferation and cytotoxicity. Cells which received no irradiation (OJ/cm~2), irradiation alone (5 J/cm~2) or treated with PS alone (no irradiation) served as controls. Results: ZnPcS_(mix) localized in both lysozomes and mitochondria in both A549 and DLD-1 cells. A549 cells treated with PDT showed a significant decrease in viability and proliferation in all PS concentrations used, while in DLD-1 cells a significant decrease was seen with concentrations of 10, 20 and 40 μM. In absence of light, ZnPcS_(mix) did not result in cellular toxicity in A549 cells whereas in DLD-1 cells it resulted in a reduction in cell proliferation only at a concentration of 40μM. Conclusion: ZnPcS_(mix) was effective in inducing cell death in both cell lines when localized in vital organelles such mitochondria and lysozomes which are essential for cell functioning. Pho-toactivated ZnPcS_(mix) affected the cells at different concentration and yielded good therapeutic results in vitro.
机译：背景：光动力疗法（PDT）是一种用于治疗癌细胞的治疗方式。先前已经表明，混合的磺化金属酞菁配合物锌磺酞菁锌（ZnPcS_（mix））可有效地破坏肺癌细胞。这项研究旨在确定ZnPcS_（mix）的亚细胞定位及其对两种癌细胞系的影响。方法：以5 J / cm〜2的波长在680 nm处活化ZnPcS_（mix）。使用了结肠癌（DLD-1）和肺癌（A549）细胞系。 ZnPcS_（mix）的亚细胞定位通过荧光显微镜确定。单独PS的毒性以及光和PS（PDT）的结合通过细胞形态，生存力，增殖和细胞毒性来确定。不接受照射（OJ / cm〜2），单独照射（5J / cm〜2）或单独接受PS处理（不照射）的细胞作为对照。结果：ZnPcS_（mix）位于A549和DLD-1细胞的溶酶体和线粒体中。在所有使用的PS浓度下，用PDT处理的A549细胞均显示出活力和增殖能力的显着降低，而在DLD-1细胞中，浓度分别为10、20和40μM时，则显着降低。在没有光照的情况下，ZnPcS_（mix）在A549细胞中不会导致细胞毒性，而在DLD-1细胞中，仅在40μM的浓度下它会导致细胞增殖减少。结论：ZnPcS_（mix）定位于两个重要细胞器的线粒体和溶酶体等重要细胞器中时，可有效诱导两种细胞的细胞死亡，这对细胞功能至关重要。光活化的ZnPcS_（mix）以不同的浓度影响细胞，并在体外产生良好的治疗效果。

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