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首页> 外文期刊>Photodiagnosis and photodynamics therapy >Validation of a non-invasive fluorescence imaging system to monitor dermatological PDT
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Validation of a non-invasive fluorescence imaging system to monitor dermatological PDT

机译：验证无创荧光成像系统以监测皮肤病学PDT

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Background: Methyl-aminolevulinate (AAAL) photodynamic therapy (PDT) involves selective accumulation of a photosensitiser, protoporphyrin IX (PplX), primarily in tumour tissue, which in combination with visible light and tissue oxygen results in reactive oxygen species (ROS) production and thus cellular destruction.rnMethods: A non-invasive fluorescence imaging system (Dyaderm, Biocam, Germany) has been employed to acquire colour (morphological) and fluorescent (physiological) images simultaneously during dermatological PDT. This system had been previously utilised for fluorescence diagnosis, however, here changes in PplX concentration within the skin lesions and normal tissue were followed after AAAL application. Measurements were also recorded from a synthetic PplX standard.rnResults: Results indicated that imaging distance, imaging angle, position of the region of interest and light conditions all altered the PplX levels acquired from the synthetic PplX standard. The imaging system was therefore adapted and a standard operating procedure developed allowing reproducible images of dermatological lesions to be acquired. Different concentrations of synthetic PplX were analysed with the system and a linear relationship was observed between the PplX concentration and the mean greyscale value calculated for the images acquired up torn10 μM.rnConclusions: The Dyaderm imaging system can now be used reproducibly with confidence to semi-quantify PplX (within the range of 0-10 μM) within dermatological lesions using the standard operating procedure derived from this work.
机译：背景：氨基乙酰丙酸甲酯（AAAL）光动力疗法（PDT）涉及光敏剂原卟啉IX（PplX）的选择性积累，主要是在肿瘤组织中，与可见光和组织氧结合会导致活性氧（ROS）的产生和方法：采用非侵入式荧光成像系统（Dyaderm，Biocam，德国）在皮肤病学PDT中同时采集彩色（形态）和荧光（生理）图像。该系统先前已被用于荧光诊断，但是，在此应用AAAL后，要跟踪皮肤病变和正常组织中PplX浓度的变化。结果：结果表明，成像距离，成像角度，感兴趣区域的位置和光照条件都改变了从合成PplX标准获得的PplX水平。因此，对成像系统进行了调整，并开发了标准操作程序，可以获取皮肤病灶的可再现图像。用该系统分析了不同浓度的合成PplX，并观察到PplX浓度与为获取至10μM的图像计算的平均灰度值之间存在线性关系。结论：Dyaderm成像系统现在可以放心地重用到半使用这项工作衍生的标准操作程序对皮肤病灶内的PplX（0-10μM范围内）进行定量。

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