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首页> 外文期刊>Photodiagnosis and photodynamics therapy >Induction of early apoptosis in human nasopharyngeal carcinoma cells by mTHPC-mediated photocytotoxicity
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Induction of early apoptosis in human nasopharyngeal carcinoma cells by mTHPC-mediated photocytotoxicity

机译：mTHPC介导的光细胞毒性诱导人鼻咽癌细胞的早期凋亡

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Objectives: In this study, the early apoptotic events elicited by mTHPC-mediated photocytotoxicity were explored in a human nasopharyngeal carcinoma cell line (NPC/HK1). Methods and materials: NPC/HK1 cells (5 × 10~3) were incubated with photosensitizer mTHPC (0.8μg/ml) in chamber slides for 20 h and subjected to light irradiation at 2J/cm~2 (LD_(80)). Morphologic changes of treated cells were examined under light microscopy and confocal microscopy at 0-4 h after the light irradiation. The early stage of apoptosis was detected by fluorescein-conjugated Annexin V (Annexin V-FITC) assay. Mitochondrial membrane damage and cytochrome c release were determined by flowcytometric analysis. Bcl-2 expression was measured by Western blot analysis.rnResults: One hour after mTHPC-mediated photodynamic therapy (PDT), microscopic examination showed membrane blebbing and cell shrinkage. Annexin V-FITC assay showed that a considerable number of NPC/HK1 cells became apoptotic. Flowcytometric analysis showed that the cytochrome c was released at 1 h after PDT. Bcl-2 expression also declined significantly compared to control groups.rnConclusions: mTHPC-mediated photo-cytotoxicity can effectively induce early apoptotic responses in NPC/HK1 cells which might be modulated by mitochondrial damages and Bcl-2 inhibition.
机译：目的：在这项研究中，在人鼻咽癌细胞系（NPC / HK1）中研究了mTHPC介导的光细胞毒性引起的早期凋亡事件。方法与材料：将NPC / HK1细胞（5×10〜3）与光敏剂mTHPC（0.8μg/ ml）在室玻片中孵育20小时，并以2J / cm〜2的光照射（LD_（80））。在光照射后0-4小时，在光学显微镜和共聚焦显微镜下检查处理的细胞的形态变化。通过荧光素缀合的膜联蛋白V（Annexin V-FITC）检测来检测凋亡的早期阶段。通过流式细胞术分析确定线粒体膜损伤和细胞色素c释放。结果：mTHPC介导的光动力疗法（PDT）1小时后，镜检可见膜起泡和细胞皱缩。膜联蛋白V-FITC测定显示相当数量的NPC / HK1细胞凋亡。流式细胞仪分析表明，PDT后1小时释放了细胞色素c。结论：mTHPC介导的光细胞毒性能有效诱导NPC / HK1细胞早期凋亡反应，其可能受线粒体损伤和Bcl-2抑制作用的调节。

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