• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Evaluation of ALA-PDT of ovarian cancer in the Fisher 344 rat tumor model
【24h】

Evaluation of ALA-PDT of ovarian cancer in the Fisher 344 rat tumor model

机译：Fisher 344大鼠肿瘤模型中卵巢癌ALA-PDT的评估

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: This study aimed to evaluate aminolevulinic acid-photodynamic therapy (ALA-PDT) in an experimental tumor model to expand the use of PDT in the treatment of ovarian cancer with peritoneal carcinosis. Materials and methods: 5-Aminolevulinic acid (ALA) (Photocure ASA, Norway) 60 mg/kg was injected in the peritoneum cavity of 45 female rats Fisher with induced peritoneal metastases of ovarian cancer. ALA-PDT was performed 4 h later with two different lasers: (1) laser diode (Diomed, Cambridge, UK), at 630nm, 100 mW/cm~2, or (2) KTP laser (Laser Quantum, Stockport, UK), 532 nm, 30 mW/cm~2. The animals were divided into five groups: LASER ALONE group, CTRL group (no cancer), NO LASER group, 1 DOSE group (PDT during 1 s) and 1.5 DOSE group (PDT during 1.5 s). Biopsies were taken 24 h after treatment. A semi-quantitative score called necrosis value (NV) was assigned to each sample that reflected the depth of the peritoneal necrosis. Results: In the first two groups, the peritoneum remained intact irrespective of the wavelength. In the 1 DOSE group, necrosis was observed for 532 nm and 630 nm. In the 1.5 DOSE group, necrosis was observed for 532 nm (NV: 3.22 ±0.83) and 630 nm (NV: 2.67±1.00) (p＜0.05). The mesothelium strongly thinned out in the diffuse shape of the tumor. Conclusion: Only ALA-PDT induces tumor necrosis with either 532 nm and 630 nm and should be considered an effective therapy for micrometastasis of ovarian cancer. This preliminary study deserves further experiments.
机译：目的：本研究旨在评估氨基乙酰丙酸光动力疗法（ALA-PDT）在实验性肿瘤模型中的应用，以扩大PDT在治疗腹膜癌卵巢癌中的应用。材料和方法：将60 mg / kg的5-氨基乙酰丙酸（ALA）（挪威的Photocure ASA）注射到45只雌性大鼠Fisher诱发卵巢癌腹膜转移的腹膜腔中。 4小时后用两种不同的激光进行ALA-PDT：（1）630nm，100 mW / cm〜2的激光二极管（Diomed，Cambridge，UK）或（2）KTP激光（Laser Quantum，Stockport，UK） 532 nm，30 mW / cm〜2。将动物分为五个组：激光单独组，CTRL组（无癌症），无激光组，1个剂量组（1s期间的PDT）和1.5个剂量组（1.5s期间的PDT）。治疗后24小时进行活检。为每个反映腹膜坏死深度的样本分配一个称为坏死值（NV）的半定量评分。结果：在前两组中，无论波长如何，腹膜均保持完整。在1个DOSE组中，观察到532 nm和630 nm坏死。在1.5剂量组中，观察到532nm（NV：3.22±0.83）和630nm（NV：2.67±1.00）的坏死（p ＜0.05）。间皮在肿瘤的扩散形状中强烈变薄。结论：只有ALA-PDT可以诱导532 nm和630 nm的肿瘤坏死，应被认为是卵巢癌微转移的有效疗法。这项初步研究值得进一步的实验。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号