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首页> 外文期刊>Photodiagnosis and photodynamics therapy >PDT with TOOKAD~® studied in the chorioallantoic membrane of fertilized eggs
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PDT with TOOKAD~® studied in the chorioallantoic membrane of fertilized eggs

机译：在受精卵的绒毛膜中研究了含TOOKAD〜®的PDT

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Background: The potential application of TOOKAD~®-PDT for the treatment of blood vessels was investigated. TOOKAD~® (WST09), a novel palladium-bacteriopheophorbide absorbs light in the near IR with a high quantum yield of intersystem crossing. Our study assessed the efficacy of this drug in inducing vascular damage with a view to its possible use in the treatment of age-related macular degeneration. Methods: Vascular damage of TOOKAD~®-PDT was studied in neovessels of the chorioallantoic membrane of fertilized eggs. Pharmacokinetic investigations were done by video microscopy and laser scanning microscopy. To induce damage vessels were irradiated with 763 nm light from a diode laser. Results: TOOKAD~® was accumulated in the vessels in the first minutes following injection. TOOKAD~® fluorescence was seen predominantly in the lumen and not in the vascular endothelial layer. Although fluorescence was very weak it could be attributed to TOOKAD~® from the fluorescence spectrum in the circulation. Damage assessment was done 24 h after application of 763 nm light. No significant difference in the degree of damage was observed with different short drug-light intervals (1—10min), but damage increased with the light energy dose. Closure of smaller vessels and vanished capillaries could be achieved by irradiation with 5 J/cm~2 and a TOOKAD~® dose of 33 μg/embryo, corresponding to a phototoxic efficacy of 0.0062. Conclusions: From the results discussed in this work, TOOKAD~® could be a potential drug for the PDT of age-related macular degeneration in which the growth of new vessels in the choroids can lead to loss of vision.
机译：背景：研究了TOOKAD〜-PDT在血管治疗中的潜在应用。 TOOKAD〜®（WST09）是一种新型的钯-细菌-Phophophophorbide吸收近红外光，具有很高的跨系统量子产率。我们的研究评估了该药物在诱导血管损伤中的功效，以期可能用于治疗与年龄有关的黄斑变性。方法：在受精卵绒毛膜的新血管中研究TOOKAD〜-PDT的血管损伤。通过视频显微镜和激光扫描显微镜进行药代动力学研究。为了引起损伤，用来自二极管激光器的763nm光照射血管。结果：注射后的第一分钟，TOOKAD®积聚在血管中。主要在管腔而不是在血管内皮层中看到了TOOKAD荧光。尽管荧光非常弱，但可以归因于循环中的荧光光谱。 763 nm光照后24小时进行损伤评估。在短短的药物-光照间隔（1-10min）下，观察到的损伤程度没有显着差异，但是损伤随着光能剂量的增加而增加。通过以5 J / cm〜2的辐照量和33μg/胚胎的TOOKAD®剂量辐照，可以封闭较小的血管和消失的毛细管，相当于0.0062的光毒性。结论：从这项工作中讨论的结果来看，TOOKAD〜®可能是老年性黄斑变性PDT的潜在药物，其中脉络膜中新血管的生长会导致视力丧失。

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