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The influence of intracellular mTHPC concentration upon photobleaching dynamics

机译：细胞内mTHPC浓度对光漂白动力学的影响

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In this study, we report the effect of the local photosensitizer concentration upon the dynamics of the singlet oxygen-mediated photobleaching, within formalin-fixed keratinocytes. Although the cells were incubated at a single mTHPC dose, cell-to-cell variations in concentration were defined within the perinuclear region by differences in the initial amplitude of the laser-induced fluorescence emission, located around 652 nm. At a fixed laser fluence-rate, it was found that the photobleaching, when plotted as a function of delivered light dose, proceeded more rapidly at higher drug concentration. The mTHPC spectral emission profile is shown to be approximately Lorentzian and remains unchanged as the photobleaching proceeds. This indicates that there is no perturbation of the detected signal due to the inner-filter effect.
机译：在这项研究中，我们报告了局部光敏剂浓度对福尔马林固定的角质形成细胞内单线态氧介导的光漂白动力学的影响。尽管细胞是在单次mTHPC剂量下孵育的，但细胞周围浓度的变化是通过位于652 nm附近的激光诱导荧光发射的初始幅度的差异来定义的。发现在固定的激光注量速率下，当将光漂白作为递送的光剂量的函数作图时，在较高的药物浓度下光漂白进行得更快。 mTHPC光谱发射曲线显示为近似洛伦兹曲线，并且随着光漂白的进行而保持不变。这表明由于内部滤波器效应，检测到的信号没有干扰。

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