• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Antimicrobial photodynamic activity of rose bengal conjugated multi walled carbon nanotubes against planktonic cells and biofilm of Escherichia coli
【24h】

Antimicrobial photodynamic activity of rose bengal conjugated multi walled carbon nanotubes against planktonic cells and biofilm of Escherichia coli

机译：玫瑰孟加拉共轭多壁碳纳米管对浮游细胞和大肠杆菌生物膜的抗菌光动力活性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: The global threat of antimicrobial resistance especially due to the bacterial biofilms has engaged researchers in the search of new treatment modalities. Antimicrobial photodynamic inactivation (aPDI) is one of the alternative treatment modalities which kills bacteria by generating endogenous reactive oxygen species (ROS). In this work authors evaluated the antibacterial and antibiofilm efficacy of rose Bengal (RB) conjugated to CNT against E. coli.Methods: The interaction of anionic dye to the CNT was studied using UV vis spectroscopy, HRTEM, FTIR and spectrofluorometry. Phototoxicity of RBCNT conjugate against E. coli was studied using a green light of 50 mW and radiant exposure of 1674.7 J/ cm(2) for 10 min. The antibiofilm activity and mechanism of action of RBCNT conjugate in presence of light was evaluated.Results: The loading and encapsulation was found to be 15.46 +/- 1.05 and 61.85 +/- 4.23% respectively. The photodynamic inactivation of planktonic cells of E. colt was found to 5.46 and 3.56 log(10) CFU/ml reduction on treatment with RBCNT and RB respectively. The conjugate also exhibited efficient and enhanced antibiofilm activity against E. coll. The study of mechanism of action has confirmed cell membrane and DNA damage were the two main targets of aPDI.Conclusion: This report has concluded the efficient photodynamic inactivation occurred in Gram negative bacteria E. coli due to the increased production of ROS inside the bacterial cells. Hence, the newly synthesized RBCNT conjugate can be efficiently utilized to control infections caused by E. coli.
机译：背景：尤其是由于细菌生物膜引起的全球抗药性威胁已促使研究人员寻求新的治疗方法。抗菌光动力灭活（aPDI）是通过产生内源性活性氧（ROS）杀死细菌的替代治疗方式之一。在这项工作中，作者评估了与CNT结合的玫瑰孟加拉（RB）对大肠杆菌的抗菌和生物膜作用。方法：使用紫外可见光谱，HRTEM，FTIR和荧光光谱法研究了阴离子染料与CNT的相互作用。使用50 mW的绿光和1674.7 J / cm（2）的辐射暴露10分钟研究了RBCNT共轭物对大肠杆菌的光毒性。结果表明：负载量和包封率分别为15.46 +/- 1.05和61.85 +/- 4.23％。发现分别用RBCNT和RB处理后，大肠杆菌的浮游细胞的光动力失活分别降低了5.46和3.56 log（10）CFU / ml。该缀合物还显示出针对大肠杆菌的有效且增强的抗生物膜活性。作用机理的研究已经证实细胞膜和DNA损伤是aPDI的两个主要目标。结论：本报告得出结论，由于细菌细胞内ROS的产生增加，革兰氏阴性细菌大肠杆菌发生了有效的光动力学灭活。 。因此，新合成的RBCNT缀合物可以有效地用于控制由大肠杆菌引起的感染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号