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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Low power blue LED exposure increases effects of doxorubicin on MDA-MB-231 breast cancer cells
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Low power blue LED exposure increases effects of doxorubicin on MDA-MB-231 breast cancer cells

机译：低功率蓝色LED暴露会增加阿霉素对MDA-MB-231乳腺癌细胞的作用

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Patients with triple negative breast cancer can develop side effects as a result of chemotherapy. Photodynamic therapy may reduce these side effects if the chemotherapy agent could also act as a photosensitizer. Thus, the aim of this work was to evaluate cytotoxicity and reactive oxygen species production induced by doxorubicin and low power blue LED in breast cancer cultures. Cell viability and reactive oxygen species (ROS) in MDA-MB-231 cultures were evaluated in response to different doxorubicin concentrations and blue LED fluences. Compared with control, cell cultures only incubated with doxorubicin at 25 nM showed 23% of cell viability reduction while its combination with blue LED at 640 J/cm(2) reduced 40% of cell viability after 24 h. After 48 h, reduction of cell viability raises to 40% in cell cultures only incubated with doxorubicin and 55% when combined with blue LED. Evaluation 30 min after treatment showed that cells incubated with doxorubicin and exposed to blue LED generated 22% more ROS than controls. Those results show that incubation with doxorubicin combined with exposure to low power blue LED is more cytotoxic and more effective to increase ROS levels in MDA-MB-231 cultures than incubation with doxorubicin alone.
机译：三阴性乳腺癌的患者可能会因化疗而出现副作用。如果化学治疗剂还可以用作光敏剂，则光动力疗法可以减少这些副作用。因此，这项工作的目的是评估阿霉素和低功率蓝光LED在乳腺癌培养物中诱导的细胞毒性和活性氧的产生。响应不同的阿霉素浓度和蓝色LED能量密度，评估了MDA-MB-231培养物中的细胞活力和活性氧（ROS）。与对照相比，仅在25 nM下与阿霉素孵育的细胞培养物显示24％的细胞活力降低，而其与640 J / cm（2）的蓝色LED的组合在24小时后降低40％的细胞活力。 48小时后，仅与阿霉素一起孵育的细胞培养物中细胞活力下降至40％，而与蓝色LED组合时则达到55％。处理后30分钟的评估结果显示，与阿霉素孵育并暴露于蓝色LED的细胞比对照产生的ROS多22％。这些结果表明，与仅与阿霉素温育相比，与阿霉素温育相结合并暴露于低功率蓝光LED下对MDA-MB-231培养物中的ROS更具细胞毒性，更有效地提高了ROS水平。

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