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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Expression of peptide transporter 1 has a positive correlation in protoporphyrin Ⅸ accumulation induced by 5-aminolevulinic acid with photodynamic detection of non-small cell lung cancer and metastatic brain tumor specimens originating from non-small cell lung cancer
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Expression of peptide transporter 1 has a positive correlation in protoporphyrin Ⅸ accumulation induced by 5-aminolevulinic acid with photodynamic detection of non-small cell lung cancer and metastatic brain tumor specimens originating from non-small cell lung cancer

机译：肽转运蛋白1的表达与5-氨基乙酰丙酸诱导的原卟啉Ⅸ积累与光动力学检测非小细胞肺癌和源于非小细胞肺癌的转移性脑肿瘤标本呈正相关

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Background: Recently, 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX fluorescence was reported to be a useful tool during total surgical resection of high-grade gliomas. However, the labeling efficacy of protoporphyrin IX fluorescence is lower in metastatic brain tumors compared to that in high-grade gliomas, and the mechanism underlying protoporphyrin IX fluorescence in metastatic brain tumors remains unclear. Lung cancer, particularly non-small cell lung cancer (NSCLC), is the most common origin for metastatic brain tumor. Therefore, we investigated the mechanism of protoporphyrin IX fluorescence in NSCLC and associated metastatic brain tumors.Methods: Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) was employed to evaluate the protein and mRNA levels of five transporters and enzymes involved in the porphyrin biosynthesis pathway: peptide transporter 1 (PEPT1), hydroxymethylbilane synthase (HMBS), ferrochelatase (FECH), ATP-binding cassette 2 (ABCG2), and heme oxygenase 1 (HO-1). The correlation between protein, mRNA, and protoporphyrin IX levels in NSCLC cells were evaluated in vitro. Immunohistochemistry was used to determine proteins that played a key role in intraoperative protoporphyrin IX fluorescence in clinical samples from patients with NSCLC and pathologically confirmed metastatic brain tumors.Results: A significant correlation between PEPT1 expression and protoporphyrin IX accumulation in vitro was identified by western blotting (P = 0.003) and qRT-PCR (P = 0.04). Immunohistochemistry results indicated that there was a significant difference in PEPT1 between the intraoperative protoporphyrin IX fluorescence-positive and protoporphyrin IX fluorescence-negative groups (P = 0.009).Conclusion: Expression of PEPT1 was found to be positively correlated with 5-ALA-induced protoporphyrin IX accumulation detected by photodynamic reaction in metastatic brain tumors originating from NSCLC.
机译：背景：最近，据报道5-氨基乙酰丙酸（5-ALA）诱导的原卟啉IX荧光是高级别神经胶质瘤全外科切除术的有用工具。然而，与高级神经胶质瘤相比，原卟啉IX荧光在转移性脑肿瘤中的标记功效较低，并且原卟啉IX荧光在转移性脑肿瘤中的潜在机制仍不清楚。肺癌，特别是非小细胞肺癌（NSCLC），是转移性脑肿瘤的最常见起源。因此，我们研究了原卟啉IX荧光在非小细胞肺癌和相关转移性脑肿瘤中的作用机制。方法：采用蛋白质印迹和定量实时聚合酶链反应（qRT-PCR）评估涉及的5种转运蛋白和酶的蛋白质和mRNA水平在卟啉生物合成途径中：肽转运蛋白1（PEPT1），羟甲基胆碱合酶（HMBS），铁螯合酶（FECH），ATP结合盒2（ABCG2）和血红素加氧酶1（HO-1）。体外评估了NSCLC细胞中蛋白质，mRNA和原卟啉IX水平之间的相关性。免疫组织化学方法检测了在非小细胞肺癌和经病理证实的转移性脑肿瘤的临床样本中，在术中原卟啉IX荧光中起关键作用的蛋白质。结果：通过Western印迹鉴定了PEPT1表达与体外原卟啉IX积累之间的显着相关性（ P = 0.003）和qRT-PCR（P = 0.04）。免疫组织化学结果表明，术中原卟啉IX荧光阳性组和原卟啉IX荧光阴性组之间的PEPT1存在显着差异（P = 0.009）。结论：发现PEPT1的表达与5-ALA诱导的原卟啉呈正相关。通过光动力反应在源自NSCLC的转移性脑肿瘤中检测到IX积累。

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