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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >The impact of Aggregatibacter actinomycetemcomitans biofilm-derived effectors following antimicrobial photodynamic therapy on cytokine production in human gingival fibroblasts
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The impact of Aggregatibacter actinomycetemcomitans biofilm-derived effectors following antimicrobial photodynamic therapy on cytokine production in human gingival fibroblasts

机译：抗菌光动力疗法对人牙龈成纤维细胞中放线杆菌细菌膜生物效应物的影响

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Background: Antimicrobial photodynamic therapy (aPDT) is an effective adjunctive therapeutic modality for the treatment of local infections, including periodontitis and peri-implantitis. After receiving aPDT, microbial cells in the biofilm structure may produce and/ or release soluble biofilm-derived effectors (BDEs), which may affect the biology of the host cells in the community context of their surrounding microenvironment. Given the fact that no study has investigated the role of BDEs following aPDT in the pathogenesis of infectious diseases, the aim of the current study was to determine the effect of BDEs of Aggregatibacter actinomycetemcomitans following exposure to sub-lethal doses of indocyanine green (ICG)-aPDT on human gingival fibroblasts (HGFs) in terms of cytokines produced.Materials and methods: In this study, we evaluated the effect of biofilm-conditioned medium (BCM) resulting from the treatment of A. actinomycetemcomitans biofilm with a sub-lethal dose of aPDT on cytokines production, including IL-6, IL-8, CXCL10, TGF-beta, and bFGF of HGFs using enzyme-linked immunoassays (ELISA). The sensitivity of cytokines to BDEs was determined by micro-titer plates.Results: The maximal sub-lethal dose of ICG-PDT was 20.15 mu M/mL ICG at a fluence of 31.2 J/cm(2). The BCM of ICG-PDT-treated viable A. actinomycetemcomitans significantly reduced IL-6, IL-8, and CXCLIO levels compared to the BCM of untreated viable A. actinomycetemcomitans (78-, 93-, and 61.6-fold reduction, respectively; all P < 0.01). TGF-beta and bFGF were strongly induced by BCM of ICG-PDT treated viable A. actinomycetemcomitans (by 57.7 and 36.1 folds, respectively; both P < 0.05). The BCM of untreated viable A. actinomycetemcomitans degraded most of the CxCL10, TGF-beta and bFGF (58.8, 61.5, and 71.6%, respectively) in 24 h, while it degraded 9.3% of IL-6 and 15.1% of IL-8 after 24 h.Conclusion: The results of the current study revealed that a sub-lethal dose of ICG-aPDT through the effect of BCM on HGFs could not only significantly reduce the production of pro-inflammatory cytokines but also promoted their role in periodontal regeneration due to increasing the bFGF level. Altogether, ICG-aPDT, with it's antimicrobial effects reduces inflammation and induces of tissue regeneration resulting from BCM, can be considered an efficient adjunctive therapeutic method for the treatment of local infections.
机译：背景：抗菌素光动力疗法（aPDT）是一种有效的辅助治疗方式，用于治疗局部感染，包括牙周炎和种植体周围炎。接受aPDT后，生物膜结构中的微生物细胞可能产生和/或释放可溶性生物膜衍生的效应物（BDE），这可能会影响宿主细胞在其周围微环境的群落环境中的生物学特性。鉴于尚无研究调查aPDT后BDEs在传染病发病机理中的作用这一事实，本研究的目的是确定暴露于亚致死剂量的吲哚花青绿（ICG）后放线放线杆菌的BDEs的作用。 -aPDT对人牙龈成纤维细胞（HGFs）产生的细胞因子的影响。材料与方法：在这项研究中，我们评估了亚致死剂量亚放线杆菌对生物膜条件培养基（BCM）的治疗作用。酶联免疫法（ELISA）检测aPDT对细胞因子产生的影响，包括HGF的IL-6，IL-8，CXCL10，TGF-beta和bFGF。结果：用31.2 J / cm（2）的通量，ICG-PDT的最大致死剂量为20.15μM / mL ICG。与未经处理的活放线放线杆菌的BCM相比，ICG-PDT处理的活放线放线杆菌的BCM显着降低了IL-6，IL-8和CXCLIO水平（分别降低了78倍，93倍和61.6％；所有P <0.01）。 TGF-β和bFGF被ICG-PDT治疗的活放线放线杆菌的BCM强烈诱导（分别降低了57.7和36.1倍；两者均P <0.05）。未经处理的活放线放线杆菌的BCM在24小时内降解了大多数CxCL10，TGF-beta和bFGF（分别为58.8、61.5和71.6％），而其降解了9.3％的IL-6和15.1％的IL-8。 24小时后结论：目前的研究结果表明，通过BCM对HGF的作用，亚致死剂量的ICG-aPDT不仅可以显着减少促炎细胞因子的产生，还可以促进它们在牙周再生中的作用由于增加了bFGF水平。总而言之，ICG-aPDT具有抗微生物作用，可减少炎症并诱导BCM引起的组织再生，可被视为治疗局部感染的有效辅助治疗方法。

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