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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Assessment of sensitivity of selected Candida strains on antimicrobial photodynamic therapy using diode laser 635 nm and toluidine blue - In vitro research
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Assessment of sensitivity of selected Candida strains on antimicrobial photodynamic therapy using diode laser 635 nm and toluidine blue - In vitro research

机译：使用635 nm二极管激光和甲苯胺蓝评估所选念珠菌菌株对抗菌光动力治疗的敏感性-体外研究

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Background: Photodynamic therapy is believed to be a promising treatment for Candida infections. This study evaluated the efficacy of antimicrobial photodynamic therapy (aPDT) using the 635 nm diode laser light and toluidine blue (TB) in the elimination of selected Candida species cultured on acrylic surface.Methods: 108 acrylic plates (Methyl Methacrylate Polymer, routinely used for the production of prosthetic dentures) were placed in three sterile Petri dishes and poured with prepared suspensions of Candida strains: C. albicans, C. glabrata, and C. krusei. After all procedures of fungi incubation, fungal biofilm was visible on the plates' surfaces. The acrylic plates were divided into nine study groups (B) and nine control groups (K) for further experiments. In the study groups, the acrylic plates with fungal biofilm were immersed in TB and afterwards laser irradiation was applicated with different exposure parameters (groups: B1 - 400 mW, 24 J/cm(2), 30 s; B2 - 300 mW, 18 J/cm(2), 30 s; B3 - 200 mW, 12 J/cm(2), 30 s) separately for each Candida species. The control groups contained following parameters: no exposure to laser light or TB, treatment only with TB without laser irradiation, or only laser irradiation without previous immersion in TB. Calculations of colony forming units (CFUs) were conducted by using aCOlyte (Synbiosis). Differences in CFUs were analyzed by the Wilcoxon test.Results: In all study groups, the reduction in CFUs was statistically significant. The differences in CFUs before and after intervention were insignificant. The K3 (c.a.) control group showed a statistical reduction of Candida albicans after laser irradiation.Conclusion: Our study confirmed the efficacy of aPDT against C. albicans, C. glabrata and C. krusei being dependent on the laser parameters and the type of fungus. The advantage of this study is the validation of aPDT effectiveness in in vitro studies to transpose this data into future clinical trials using photodynamic therapy in the treatment of oral candidiasis.
机译：背景：光动力疗法被认为是念珠菌感染的一种有前途的疗法。这项研究评估了使用635 nm二极管激光和甲苯胺蓝（TB）进行的抗菌光动力疗法（aPDT）消除在丙烯酸表面上培养的某些念珠菌种类的功效。方法：108个丙烯酸板（甲基丙烯酸甲酯聚合物，通常用于将假牙的生产放入三个无菌的陪替氏培养皿中，并倒入念珠菌菌株的制备悬液：白色念珠菌，光滑念珠菌和克鲁斯念珠菌。在所有的真菌温育步骤之后，在平板表面上可见真菌生物膜。将丙烯酸板分为9个研究组（B）和9个对照组（K）进行进一步的实验。在研究组中，将带有真菌生物膜的丙烯酸板浸入TB中，然后以不同的暴露参数施加激光照射（组：B1-400 mW，24 J / cm（2），30 s; B2-300 mW，18 J / cm（2），30 s; B3-200 mW，12 J / cm（2），30 s）分别针对每个念珠菌物种。对照组包含以下参数：不暴露于激光或TB，仅在不照射激光的情况下用TB进行治疗，或仅在不事先浸入TB的情况下进行激光照射。菌落形成单位（CFUs）的计算是通过使用aCOlyte（Synbiosis）进行的。结果：在所有研究组中，CFU的减少均具有统计学意义。干预前后CFU的差异不明显。 K3（ca）对照组在激光照射后显示白色念珠菌的统计减少。结论：我们的研究证实aPDT对抗白色念珠菌，光滑念珠菌和克鲁斯氏菌的功效取决于激光参数和真菌类型。 。这项研究的优势是在体外研究中验证了aPDT的有效性，该研究将使用光动力疗法治疗口腔念珠菌病将此数据转化为未来的临床试验。

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