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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Natural photosensitizers in photodynamic therapy: In vitro activity against monolayers and spheroids of human colorectal adenocarcinoma SW480 cells
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Natural photosensitizers in photodynamic therapy: In vitro activity against monolayers and spheroids of human colorectal adenocarcinoma SW480 cells

机译：光动力疗法中的自然光敏剂：对抗单层和人结肠直肠腺癌SW480细胞的体外活动

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摘要

Photodynamic Therapy (PDT), is a treatment option for cancer.It involves the photochemical interaction of light, photosensitizer (PS) and molecular oxygen to produce radical species as well as singlet oxygen which induce cell death. Anthraquinones (AQs) have been extensively studied with respect to their UV/Vis absorption characteristics and their photosensitizing properties in photodynamic reactions. We study the photoactivity of different natural AQs (Parietin, Soranjidiol and Rubiadin) in treating monolayers and multicellular tumor spheroids (MCTSs). Rubiadin and soranjidiol were isolated and purified from the stem and leaves of Heterophyllae pustulata, and PTN was from the liquen Teloschistes flavicans by using repeated combination of several chromatographic techniques. Monolayer and spheroids of human colorectal adenocarcinoma SW480 cells were incubated with different concentrations of the AQs and then irradiated at room temperature. 24 h post-PDT cell viability, nuclear morphology and type of cell death were analyzed. We observed that Soranjidiol and Rubiadin showed no significant difference in the photosensitizing ability on monoculture of colon cancer cells (LD80 at 50 mu M and 10 J/cm(2), for both AQs). Nevertheless, for Parietin (PTN) LD80 was achieved at (20 mu M using the same light dose (10 J/cm(2)). The death mechanism induced post-PDT was necrosis by use of Soranjidol and Rubiadin and apoptosis by use of PTN. Furthermore, in MCTSs of 300 and 900 mu m, the treatment PTN- PDT produces the greatest cytotoxic effect. The three AQs analyzed could be promising chemotherapeutic candidates as anticancer PDT agents.
机译：光动力疗法（PDT）是癌症的治疗选择。涉及光，光敏剂（PS）和分子氧的光化学相互作用，以产生根本物种以及诱导细胞死亡的单次氧。在光动力反应中，已经广泛地研究了蒽醌（AQS）和它们在光动力反应中的光敏性能。我们研究不同的天然AQS（野生素，索兰二醇和Rubiadin）治疗单层和多细胞肿瘤球状体（MCTS）的光度。将Rubiadin和Soranjidiol分离并从Heterophyllae Pustulata的茎和叶中纯化，并且通过使用多种色谱技术的重复组合来自Liquen Teloschistes Flavicans的PTN。将人结肠直肠腺癌的单层和球状细胞与不同浓度的AQ一起温育，然后在室温下照射。分析了24小时PDT后细胞活力，核形态和细胞死亡类型。我们观察到Soranjidiol和Rubiadin在结肠癌细胞的单一培养中没有显着差异（LD80在50μm和10J / cm（2），两个aqs）。然而，对于野生素（PTN）LD80（使用相同的光剂量（10J / cm（2））达到（20μm）。通过使用Soranjidol和Rubiadin和通过使用的细胞凋亡，诱导PDT的死亡机制是坏死PTN。此外，在MCTS为300和900 mu m中，治疗PTN-PDT产生最大的细胞毒性效果。分析的三个AQS可能是有前途的化学治疗候选者作为抗癌PDT剂。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2020年第9期|101852.1-101852.12|共12页
  • 作者

    Sol Cogno Ingrid; Gilardi Pamela; Comini Laura; Nunez-Montoya Susana C.; Luis Cabrera Jose; Alicia Rivarola Viviana;

  • 作者单位

    Univ Nacl Rio Cuarto Fac Ciencias Exactas Fisicoquim & Nat Dept Biol Mol RA-5800 Cordoba Argentina;

    Univ Nacl Rio Cuarto Fac Ciencias Exactas Fisicoquim & Nat Dept Biol Mol RA-5800 Cordoba Argentina;

    Univ Nacl Cordoba Fac Ciencias Quim Dept Ciencias Farmaceut Ciudad Univ X5000HUA Cordoba Argentina|Gobierno Prov Cordoba Ctr Excelencia Prod & Proc Cordoba Pabellon CEPROCOR Cordoba Argentina;

    Univ Nacl Cordoba Fac Ciencias Quim Dept Ciencias Farmaceut Ciudad Univ X5000HUA Cordoba Argentina|Consejo Nacl Invest Cient & Tecn Inst Multidisciplinario Biol Vegetal IMBIV Av Velez Sarsfield 1666 X5016GCN Cordoba Argentina;

    Consejo Nacl Invest Cient & Tecn Inst Multidisciplinario Biol Vegetal IMBIV Av Velez Sarsfield 1666 X5016GCN Cordoba Argentina;

    Univ Nacl Rio Cuarto Fac Ciencias Exactas Fisicoquim & Nat Dept Biol Mol RA-5800 Cordoba Argentina;

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  • 正文语种 eng
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  • 关键词

    Photodynamic therapy; Anthraquinones; Multicellular tumor spheroids; Cancer;

    机译：光动力疗法;蒽醌;多细胞肿瘤球状体;癌症;

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