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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Exposure to diode laser (810nm) affects the bacterial adherence and biofilm formation in a E. faecalis biofilm model
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Exposure to diode laser (810nm) affects the bacterial adherence and biofilm formation in a E. faecalis biofilm model

机译：暴露于二极管激光器（810nm）会影响大肠病生物膜模型中的细菌粘附和生物膜形成

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摘要

Background: A large number of studies have shown that diode laser can effectively kill Enterococcus faecalis (E. faecalis). However, to our knowledge there has been little information regarding high-level analysis of sterilization mechanism on E. faecalis biofilm models after laser irradiation. This study provides emphasis on the E. faecalis viability and exopolysaccharide content after laser irradiation. It also aims to examine whether diode laser affects the biofilm formation and adherence of E. faecalis biofilm at the level of gene expression.Methods: E. faecalis (ATCC 29,212) was inoculated for biofilm formation. After growing for 48 h, the biofilms were submitted to the following treatments, twice daily (n = 6): (i) Diode laser with energy densities as 28.7 J/ cm(2); (ii) 1% sodium hypochlorite (NaOCl) was used as a positive control; (iii) 0.9 % physiological saline (NaCl) was used as a negative control. After 120 h of biofilm growth, bactericidal activity of diode laser was evaluated by using plate count method and scanning electron microscopy (SEM). The distribution of extracellular polysaccharide was assessed by Confocal laser scanning microscopy (CLSM). Anthrone method was used to quantify the amount of water-soluble exopolysaccharide (WSE) and water-insoluble exopolysaccharides (WIE) in E. faecalis. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to assess transcription of genes (gelE, ace and esp) related to formation and adherence of E. faecalis biofilm.Results: Compared with NaCl, diode laser significantly destabilizedE. faecalis biofilm, which showing a decrease in the number of bacteria and inhibiting the adherence of biofilm (p 0.05). From Anthrone method and CLSM, the study found that diode laser lowered the concentration of WSE and WIE in biofilms (p 0.05). In addition, transcription of gene gelE, ace and esp were also reduced after irradiation of diode laser (p 0.05).Conclusion: By inhibiting the synthesis of WIE and WSE, diode laser may reduce the formation of E. faecalis biofilm. The expression of specific genes (gelE, ace and esp) involved in bacterial adherence and biofilm formation were down regulated under the exposure to diode laser (810 nm), which in turn is expected to decrease the pathogenicity of E. faecalis.
机译：背景：大量研究表明二极管激光可以有效地杀死肠球菌（粪便）。然而，对于我们的知识，在激光照射后，一直有关于E. Faecalis生物膜模型对灭菌机制的高级别分析的信息很少。该研究重点是激光照射后的E.粪便活力和外偶乙烯含量。它还旨在检查二极管激光是否会影响基因表达水平的生物膜形成和副粪便生物膜的粘附。方法：E.粪便（ATCC 29,212）被接种用于生物膜形成。生长48小时后，将生物膜提交至下列处理，每日两次（n = 6）：（i）二极管激光，能量密度为28.7J / cm（2）; （ii）1％次氯酸钠（NaOCl）用作阳性对照; （iii）0.9％的生理盐水（NaCl）用作阴性对照。在生物膜生长120小时后，通过使用板数法和扫描电子显微镜（SEM）来评价二极管激光的杀菌活性。通过共聚焦激光扫描显微镜（CLSM）评估细胞外多糖的分布。 Anthrone方法用于量化水溶性外偶（WSE）和水不溶性外碳（WIE）在大肠杆菌中的量。实时定量聚合酶链反应（RT-QPCR）用于评估与E. Faecalis Biofilm的形成和粘附相关的基因（凝胶，ACE和eSP）的转录结果：与NaCl相比，二极管激光显着稳定失效。粪便生物膜，显示细菌数量和抑制生物膜的粘附性（P <0.05）。从蒽酮方法和CLSM中，研究发现二极管激光降低了生物膜中WES和WIE的浓度（P <0.05）。此外，在二极管激光照射后，基因凝胶，ACE和ESP的转录也降低了（P <0.05）。结论：通过抑制WIE和WSE的合成，二极管激光可以减少E. Faecalis生物膜的形成。在暴露于二极管激光器（810nm）的情况下，参与细菌粘附和生物膜形成的特定基因（凝胶，ACE和esp）的表达被调节，这又预期降低了粪便的致病性。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2020年第9期|101772.1-101772.6|共6页
  • 作者

    Xiao Baiyan; Zou Zhaohui; Bhandari Junu; Zhang Yu; Yan Guohui;

  • 作者单位

    Tianjin Med Univ Sch Stomatol Hosp Stomatol 12 Observ Rd Tianjin 300070 Peoples R China;

    Tianjin Med Univ Sch Stomatol Hosp Stomatol 12 Observ Rd Tianjin 300070 Peoples R China;

    Tianjin Med Univ Sch Stomatol Hosp Stomatol 12 Observ Rd Tianjin 300070 Peoples R China;

    Tianjin Med Univ Sch Stomatol Hosp Stomatol 12 Observ Rd Tianjin 300070 Peoples R China;

    Tianjin Med Univ Sch Stomatol Hosp Stomatol 12 Observ Rd Tianjin 300070 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Diode laser; E faecalis biofilm; Adherence; Gene expression;

    机译：二极管激光;e粪菌生物膜;依从性;基因表达;

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