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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Enhanced wound healing and osteogenic potential of photodynamic therapy on human gingival fibroblasts
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Enhanced wound healing and osteogenic potential of photodynamic therapy on human gingival fibroblasts

机译：增强伤口愈合和骨动力治疗对人牙龈成纤维细胞的造成骨质愈合

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摘要

Background: Photodynamic therapy (PDT) has shown ideal antibacterial effects in clinical treatment of periodontal diseases. However, little is known about the specific potential of PDT on human gingival fibroblasts (HGFs) especially cells in the inflamed state, which may contribute to the repairi of periodontal tissue.Methods: The effect of PDT with different concentrations of methylene blue (5 mu M, 10 mu M, 20 mu M) on cell vitality of healthy and inflamed human gingival fibroblasts was evaluated by CCK-8, and cell migration was assessed by cell scratching assay. The gene expression of interleukin-6 (IL-6), interleukin-8 (IL-8), type I collagen (Col I), fibronectin (FN) and basic fibroblast growth factor (bFGF) were measured with real-time fluorescent quantitative polymerase chain reaction. The alkaline phosphatase (ALP) production and alizarin red staining of mineralized nodules in healthy and inflamed human gingival fibroblasts was evaluated to explore the effect on osteogenic differentiation.Results: PDT with relatively low concentration of methylene blue (5 mu M) inhibited the cell vitality of inflamed human gingival fibroblasts (I-HGFs) slightly (P 0.05), but had no adverse effect on healthy human gingival fibroblasts (H-HGFs) (P 0.05). As the concentration increased, PDT with 20 mu M methylene blue had significantly negative effect on both healthy and inflamed cells. Further, PDT with 5 mu M methylene blue was observed to be able to promote the migration of HGFs especially the healthy state, and increases the expression of wound healing related genes including IL-6, COL1, FN, bFGF in healthy and inflamed HGFs (P 0.05). PDT with 5 mu M methylene blue was also capable of increasing the production of ALP and mineralized nodules (P 0.05), although the better effect was observed in the laser treatment group.Conclusions: The relatively low concentration of methylene blue mediated PDT is conducive to the growth of H-HGFs while inhibiting the I-HGFs, and it also has the potential to promote the wound healing and osteogenic related functions of both healthy and inflamed HGFs.
机译：背景：光动力疗法（PDT）显示了在牙周病临床治疗中的理想抗菌作用。然而，关于人牙龈成纤维细胞（HGF）的特异性潜力尤其是发炎状态下的细胞的特异性潜力几乎熟知，这可能有助于牙周组织的修复方法。方法：PDT与不同浓度的亚甲蓝的影响（5μm通过CCK-8评估了健康和发炎的人牙龈细胞的细胞活力的M，10μm，20μm），并通过细胞划伤测定评估细胞迁移。用实时荧光定量测量白细胞介素-6（IL-8），Inter kin-8（IL-8），I型胶原蛋白（COL I），纤维素（Fn）和碱性成纤维细胞生长因子（BFGF）的基因表达聚合酶链反应。评价碱性磷酸酶（ALP）生产和茜素红染色在健康和发炎的人牙龈细胞中的矿化结节以探讨对骨质发生分化的影响。结果：具有相对低浓度的亚甲基蓝（5μm）的PDT抑制细胞生命力略微发炎人牙龈成纤维细胞（I-HGF）（P <0.05），但对健康人牙龈成纤维细胞（H-HGFS）没有不利影响（P <0.05）。随着浓度的增加，具有20μm甲基蓝的PDT对健康和发炎的细胞具有显着的负面影响。此外，观察到具有5μm的亚甲基蓝色的PDT，以促进HGFS尤其是健康状态的迁移，并增加了伤口愈合相关基因，包括IL-6，COL1，Fn，BFGF，健康和发炎的HGF（ P <0.05）。 PDT用5μm甲基蓝色也能够增加AlP和矿化结节的产生（P <0.05），但在激光处理组中观察到更好的效果。结论：相对低的亚甲基蓝介导的PDT是有利的为了抑制I-HGF的同时，H-HGFS的生长，它还具有促进健康和发炎的HGF的伤口愈合和骨质发生相关功能。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2020年第12期|101967.1-101967.9|共9页
  • 作者

    Yang Ruqian; Guo Shujuan; Xiao Shimeng; Ding Yi;

  • 作者单位

    Sichuan Univ Dept Periodont West China Coll Stomatol Chengdu Peoples R China|Sichuan Univ Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu 610041 Peoples R China;

    Sichuan Univ Dept Periodont West China Coll Stomatol Chengdu Peoples R China|Sichuan Univ Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu 610041 Peoples R China;

    Sichuan Univ Dept Periodont West China Coll Stomatol Chengdu Peoples R China|Sichuan Univ Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu 610041 Peoples R China;

    Sichuan Univ Dept Periodont West China Coll Stomatol Chengdu Peoples R China|Sichuan Univ Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu 610041 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Photodynamic therapy; Human gingival fibroblasts; Cell proliferation; Wound healing;

    机译：光动力疗法;人牙龈成纤维细胞;细胞增殖;伤口愈合;

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