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首页> 外文期刊>Plant and Cell Physiology >Apoplastic Glycosidases Active Against Xyloglucan Oligosaccharides of Arabidopsis thaliana
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Apoplastic Glycosidases Active Against Xyloglucan Oligosaccharides of Arabidopsis thaliana

机译：对抗拟南芥木葡聚糖寡糖的质外性糖苷酶

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摘要

All four glycanases necessary for the degradation of xyloglucan oligosaccharides (α-fucosidase, α-xylosidase, β-galactosidase and β-glucosidase) were found in the apoplastic fluid of Arabidopsis thaliana. These activities acted cooperatively on xyloglucan oligosaccharides (XLFG), leading to the sequential formation of XXFG, XXLG, XXXG, GXXG and XXG, as identified by matrix-assisted laser desorption ionization time of flight (MALDI-TOF). AtFXG1 (At1g67830) and AtXYL1 (At1g68560) had been previously identified as the Arabidopsis genes coding for α-fucosidase and α-xylosidase, respectively. As for the genes coding for β-galactosidase activity, we identified in phylogenetic trees 12 candidates from family 35 of glycoside hydrolases. Similarly, four genes from family 3 were selected as possible β-glucosidases active on xyloglucan. The expression level of all the selected genes was studied in different plant regions (young and mature rosette leaves, apical and basal region of the inflorescence stem, roots, flower and siliques) using quantitative real-time reverse transcription–PCR. The expression patterns were very diverse as well as their relationship with growth rates, showing a very complex situation. This could lead to highly varying proportions of the different xyloglucan oligosaccharides in different plant regions and developmental stages.
机译：在拟南芥的质外体流体中发现了降解木葡聚糖寡糖所需的所有四种聚糖酶（α-岩藻糖苷酶，α-木糖苷酶，β-半乳糖苷酶和β-葡萄糖苷酶）。这些活性协同作用于木葡聚糖寡糖（XLFG），导致XXFG，XXLG，XXXG，GXXG和XXG的顺序形成，如基质辅助激光解吸电离飞行时间（MALDI-TOF）所鉴定。 AtFXG1（At1g67830）和AtXYL1（At1g68560）先前已被分别鉴定为编码α-岩藻糖苷酶和α-木糖苷酶的拟南芥基因。至于编码β-半乳糖苷酶活性的基因，我们在系统发育树中鉴定了糖苷水解酶35家族的12个候选基因。同样，从家族3中选择了四个基因作为可能对木葡聚糖具有活性的β-葡萄糖苷酶。使用定量实时逆转录-PCR研究了所有选定基因在不同植物区域（幼小和成熟的莲座丛叶，花序茎的根部和基部区域，根，花和长角果）的表达水平。表达模式非常多样化，并且与增长率之间的关系也非常复杂。这可能导致不同植物区域和发育阶段的不同木葡聚糖寡糖比例差异很大。

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