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首页> 外文期刊>Plant and Cell Physiology >Functional Analysis of Rice DREB1/CBF-type Transcription Factors Involved in Cold-responsive Gene Expression in Transgenic Rice
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Functional Analysis of Rice DREB1/CBF-type Transcription Factors Involved in Cold-responsive Gene Expression in Transgenic Rice

机译：水稻DREB1 / CBF型转录因子参与转基因水稻冷响应基因表达的功能分析

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The transcription factors dehydration-responsive element-binding protein 1s (DREB1s)/C-repeat-binding factors (CBFs) specifically interact with the DRE/CRT cis-acting element and control the expression of many stress-inducible genes in Arabidopsis. The genes for DREB1 orthologs, OsDREB1A and OsDREB1B from rice, are induced by cold stress, and overexpression of DREB1 or OsDREB1 induced strong expression of stress-responsive genes in transgenic Arabidopsis plants, resulting in increased tolerance to high-salt and freezing stresses. In this study, we generated transgenic rice plants overexpressing the OsDREB1 or DREB1 genes. These transgenic rice plants showed not only growth retardation under normal growth conditions but also improved tolerance to drought, high-salt and low-temperature stresses like the transgenic Arabidopsis plants overexpressing OsDREB1 or DREB1. We also detected elevated contents of osmoprotectants such as free proline and various soluble sugars in the transgenic rice as in the transgenic Arabidopsis plants. We identified target stress-inducible genes of OsDREB1A in the transgenic rice using microarray and RNA gel blot analyses. These genes encode proteins that are thought to function in stress tolerance in the plants. These results indicate that the DREB1/CBF cold-responsive pathway is conserved in rice and the DREB1-type genes are quite useful for improvement of stress tolerance to environmental stresses in various kinds of transgenic plants including rice.
机译：转录因子脱水反应元件结合蛋白1s（DREB1s）/ C重复结合因子（CBFs）与DRE / CRT顺式作用元件特异性相互作用，并控制拟南芥中许多应激诱导基因的表达。水稻的DREB1直系同源基因OsDREB1A和OsDREB1B受冷胁迫诱导，DREB1或OsDREB1的过表达诱导了转基因拟南芥植物中胁迫响应基因的强表达，从而提高了对高盐和冰冻胁迫的耐受性。在这项研究中，我们生成了过表达OsDREB1或DREB1基因的转基因水稻植物。这些转基因水稻植物不仅显示出在正常生长条件下的生长迟缓，而且还提高了对干旱，高盐和低温胁迫的耐受性，例如过表达OsDREB1或DREB1的转基因拟南芥植物。与转基因拟南芥植物一样，我们还检测到转基因水稻中渗透保护剂（例如游离脯氨酸和各种可溶性糖）的含量升高。我们使用微阵列和RNA凝胶印迹分析在转基因水稻中鉴定了OsDREB1A的目标应激诱导基因。这些基因编码被认为在植物的胁迫耐受性中起作用的蛋白质。这些结果表明DREB1 / CBF冷应答途径在水稻中是保守的，并且DREB1型基因对于改善包括水稻在内的各种转基因植物对环境胁迫的胁迫耐受性非常有用。

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  • 来源
    《Plant and Cell Physiology》 |2006年第1期|141-153|共13页
  • 作者

    Yusuke Ito; Koji Katsura; Kyonoshin Maruyama; Teruaki Taji; Masatomo Kobayashi; Motoaki Seki; Kazuo Shinozaki; and Kazuko Yamaguchi-Shinozaki;

  • 作者单位

    Biological Resources Division Japan International Research Center for Agricultural Sciences (JIRCAS) Tsukuba Ibaraki 305-8686 Japan;

    Laboratory of Plant Molecular Biology RIKEN Tsukuba Institute Tsukuba Ibaraki 305-0074 Japan;

    Experimental Plant Division BioResource Center RIKEN Tsukuba Institute Tsukuba Ibaraki 305-0074 Japan;

    Core Research for Evolutional Science and Technology (CREST) Japan Science and Technology Agency Kawaguchi Saitama 332-0012 Japan;

    Graduate School of Agricultural and Life Sciences The University of Tokyo Bunkyo Tokyo 113-8657 Japan;

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