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首页> 外文期刊>Plant and Cell Physiology >AtVAM3 is Required for Normal Specification of Idioblasts, Myrosin Cells
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AtVAM3 is Required for Normal Specification of Idioblasts, Myrosin Cells

机译：正常规格的成肌细胞，黑芥子细胞需要AtVAM3

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Myrosin cells in Capparales plants are idioblasts that accumulate thioglucoside glucohydrolase (TGG, also called myrosinase), which hydrolyzes glucosinolates to produce toxic compounds for repelling pests. Here, we show that AtVAM3 is involved in development of myrosin cells. It has been shown that yeast VAM3 is a Qa-SNARE that is involved in vesicle transport of vacuolar proteins and vacuolar assembly. We found that two Arabidopsis atvam3 alleles, atvam3-3 and atvam3-4/ssm, accumulate large amounts of TGG1 and TGG2 that are enzymatically active. An immunogold analysis revealed that TGGs were specifically localized in the vacuole of myrosin cells in atvam3 mutants. This result indicates that TGGs are normally transported to vacuoles in these mutants and that AtVAM3 is not essential for vacuolar transport of the proteins. We developed a staining method with Coomassie brilliant blue that detects myrosin cells in whole leaves by their high TGG content. This method showed that atvam3 leaves have a larger number of myrosin cells than do wild-type leaves. Myrosin cells were scattered along leaf veins in wild-type leaves, while they were abnormally distributed in atvam3 leaves. The mutants developed a network of myrosin cells throughout the leaves: myrosin cells were not only distributed continuously along leaf veins, but were also observed independent of leaf veins. The excess of myrosin cells in atvam3 mutants might be responsible for the abnormal abundance of TGGs and the reduction of elongation of inflorescence stems and leaves in these mutants. Our results suggest that AtVAM3 has a plant-specific function in development of myrosin cells.
机译：Capparales植物中的黑芥子细胞是成纤维细胞，会积聚硫葡糖苷葡糖水解酶（TGG，也称为黑芥子酶），该酶水解芥子油苷以产生有毒化合物以驱除害虫。在这里，我们显示AtVAM3参与了黑色素细胞的发育。已经显示酵母VAM3是Q a -SNARE，其参与液泡蛋白的囊泡运输和液泡组装。我们发现两个拟南芥属atvam3等位基因atvam3-3和atvam3-4 / ssm积累了大量具有酶活性的TGG1和TGG2。免疫金分析表明，TGVs特异地位于atvam3突变体的黑芥子细胞液泡中。该结果表明，在这些突变体中，TGG通常被转运至液泡，并且AtVAM3对于蛋白质的液泡转运不是必需的。我们开发了一种考马斯亮蓝染色方法，通过高TGG含量检测全叶中的黑芥子素细胞。此方法表明atvam3叶片比野生型叶片具有更多的黑芥子素细胞。黑芥子细胞在野生型叶片中沿叶脉散布，而在atvam3叶片中异常分布。突变体在整个叶片上形成了黑色素细胞网络：黑色素细胞不仅沿叶脉连续分布，而且独立于叶脉而观察到。 atvam3突变体中过多的黑芥子细胞可能是导致这些突变体中TGGs异常丰富以及花序茎和叶伸长减少的原因。我们的结果表明AtVAM3在黑芥子素细胞的发育中具有植物特有的功能。

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