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首页> 外文期刊>Plant Cell Reports >Production of reactive oxygen species and induction of signaling pathways for the ACO gene expressions in tomato plants triggered by the volatile organic compound ether
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Production of reactive oxygen species and induction of signaling pathways for the ACO gene expressions in tomato plants triggered by the volatile organic compound ether

机译：挥发性有机化合物醚触发番茄中活性氧的产生和诱导ACO基因表达的信号通路

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Diethyl ether (ether), a volatile organic compound, is widely used as an industrial solvent and easily released to the environment. Acute exposure of tomato plants to high concentrations of ether caused young leaves to curl. Histochemical analyses revealed that superoxide anion (•O2 −) and hydrogen peroxide were formed sequentially by ether, and that •O2 − was the major ROS produced in response to ether exposure. We observed cell death by microscopic inspection of Evans blue-stained samples, following fumigation with ether for 6 h. The ethylene biosynthetic gene, 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), was induced as early as 15–30 min after ether fumigation and could be activated at ether concentration as low as 1 μL/L. Induction of ACO gene expression occurred simultaneously with ROS accumulation and coincided with the occurrence of cell death. Simultaneous treatment of tomato plants with mechanical wounding and ether induced differential expression of the ACO gene family. Ether strongly induced ACO4 and moderately induced ACO1, whereas mechanical wounding strongly induced ACO1 and slightly induced ACO4. Induction of the ACO gene family by ether occurred via different signaling pathways. While the ACO1 gene was induced via protein phosphorylation, the ACO4 gene was induced through protein dephosphorylation. Induction of ACO1 and ACO4 might be through MPK1, MPK2, MPK3, and PP2Ac1. These results suggest that the cellular responses of tomato plants to ether are different from the plant responses to ozone, and that tomato plants respond to different air pollutants through different perceptions and downstream signaling pathways.
机译：挥发性有机化合物二乙醚（醚）被广泛用作工业溶剂，并且容易释放到环境中。番茄植物急性暴露于高浓度的乙醚会导致幼叶卷曲。组织化学分析表明，超氧阴离子（• O 2 -）和过氧化氢是由醚顺序形成的，而• O 2 -是响应乙醚暴露而产生的主要ROS。我们用乙醚熏蒸6小时后，通过显微镜检查埃文斯蓝染色的样品观察到细胞死亡。乙烯熏蒸后最早的15–30分钟就诱导了乙烯生物合成基因1-氨基环丙烷-1-羧酸氧化酶（ACO），并且可以在低至1μL/ L的醚浓度下被激活。 ACO基因表达的诱导与ROS的积累同时发生，并且与细胞死亡的发生同时发生。机械损伤和乙醚诱导的番茄植物同时诱导ACO基因家族的差异表达。乙醚强烈诱导ACO4，中等诱导ACO1，而机械性损伤强烈诱导ACO1，轻微诱导ACO4。醚通过不同的信号传导途径诱导ACO基因家族。 ACO1基因是通过蛋白质磷酸化诱导的，而ACO4基因是通过蛋白质去磷酸化诱导的。 ACO1和ACO4的诱导可能是通过MPK1，MPK2，MPK3和PP2Ac1引起的。这些结果表明，番茄植物对乙醚的细胞反应不同于植物对臭氧的反应，并且番茄植物通过不同的感知和下游信号传导途径对不同的空气污染物做出反应。

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