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DNA amounts of roses ( Rosa L.) and their use in attributing ploidy levels

机译：玫瑰（Rosa L.）的DNA含量及其在倍性水平鉴定中的应用。

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The aims of the investigation were to characterise variability among the DNA amounts of roses and assess the predictability of ploidy levels from DNA amounts. Chromosome numbers in the genus Rosa range from 2n = 2x = 14 to 2n = 8x = 56 and aneuploidy is rare. Published 2C DNA amounts range from 0.78 pg in R. xanthina Lindl. and R. sericea Lindl. (2n = 2x = 14) to 2.91 pg in R. canina L. (2n = 5x = 35). In this investigation, DNA amounts were estimated by flow cytometry of leaf nuclei stained with propidium iodide, using Petroselinum crispum (2C DNA amount = 4.46 pg) as the internal calibration standard. Ploidy levels based on DNA amounts (DNA ploidy) were assigned by comparing their DNA amounts with published DNA amounts and identifying peaks and intervening discontinuities in frequency distributions of DNA amounts. 2C DNA amounts ranged from 0.83 pg in R. ecae (2x = 2x = 14) to 3.99 pg in R. acicularis (2n = 8x = 56). Differences in the 1Cx-values (2C DNA amount/ploidy values) were found among the taxonomic sections of Rosa. Ploidy levels could be confidently assigned to most species and cultivars, but the ploidy of some specimens in the section Caninae was uncertain for reasons attributed to genomic diversity and aneuploidy. Cytochimerism was detected in three cultivars of R. × alba. DNA ploidy was determined in 384 specimens representing 74 species and 5 horticultural classes.
机译：研究的目的是表征玫瑰的DNA量之间的变异性，并根据DNA量评估倍性水平的可预测性。 Rosa属中的染色体数范围从2n = 2x = 14到2n = 8x = 56，并且非整倍性很少见。 R. xanthina Lindl中已发布的2C DNA量范围为0.78 pg。和R. sericea Lindl。 （2n = 2x = 14）至2.91 pg（R.canina L.）（2n = 5x = 35）。在这项研究中，使用薯Petro（Petroselinum crispum）（2C DNA量= 4.46 pg）作为内部校准标准，通过流式细胞术用碘化丙啶染色的叶核估计了DNA的量。通过将DNA量与已公开的DNA量进行比较，并确定峰的数量和DNA量频率分布中的不连续性，来分配基于DNA量的倍性水平（DNA倍性）。 2C DNA的量范围从盲角红藻中的0.83 pg（2x = 2x = 14）到针状R.中的3.99 pg（2n = 8x = 56）。在罗莎的分类学部分中发现了1Cx值（2C DNA量/倍性值）的差异。倍性水平可以确定地分配给大多数物种和品种，但是由于基因组多样性和非整倍性的原因，犬科中的一些标本的倍性尚不确定。在三个R.×alba品种中检测到细胞嵌合现象。在384个样本中确定了DNA倍性，这些样本代表74个物种和5个园艺类别。

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