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首页> 外文期刊>Plant Cell Reports >Histological and molecular analysis of pollen–pistil interaction in clementine
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Histological and molecular analysis of pollen–pistil interaction in clementine

机译：柑桔花粉雌蕊相互作用的组织学和分子分析

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In contrast to model species, the self-incompatibility reaction in citrus has been poorly studied. It is assumed to be gametophytically determined and genetically controlled by the S-locus, which in other species encodes for glycoproteins (S-RNases) showing ribonuclease activity. To investigate pollen–pistil interaction, the pollen tube growth of two clementine varieties, ‘Comune’ (self-incompatible) and ‘Monreal’ (a ‘Comune’ self-compatible mutation) was analysed by histological assays in self- and cross-pollination conditions. Cross-pollination assays demonstrated that the mutation leading to self-compatibility in ‘Monreal’ occurred in the stylar tissues. Similar rates of pollen germination were observed in both genotypes. However, ‘Comune’ pollen tubes showed altered morphology and arrested growth in the upper style while in ‘Monreal’ they grew straight toward the ovary. Moreover, to identify genes putatively involved in pollen–pistil interaction and self-incompatibility, research based on the complementary DNA-amplified fragment length polymorphism technique was carried out to compare the transcript profiles of unpollinated and self-pollinated styles and stigmas of the two cultivars. This analysis identified 96 unigenes such as receptor-like kinases, stress-induced genes, transcripts involved in the phenylpropanoid pathway, transcription factors and genes related to calcium and hormone signalling. Surprisingly, a high percentage of active long terminal repeat (LTR) and non-LTR retrotransposons were identified among the unigenes, indicating their activation in response to pollination and their possible role in the regulation of self-incompatibility genes. The quantitative reverse trascription-polymerase chain reaction analysis of selected gene tags showed transcriptional differences between the two genotypes during pollen germination and pollen tube elongation.
机译：与模型物种相反，柑桔中的自溶反应尚未得到很好的研究。假定它是由配子体植物决定和遗传控制的S-基因座，在其他物种中，S-基因座编码显示核糖核酸酶活性的糖蛋白（S-RNase）。为了研究花粉与雌蕊的相互作用，通过自花授粉和交叉授粉的组织学分析法分析了两个柑桔品种“ Comune”（不相容）和“ Monreal”（“ Comune”自相容突变）的花粉管生长。条件。异花授粉试验表明，导致“ Monreal”中自我适应的突变发生在针状组织中。在两种基因型中观察到相似的花粉发芽率。但是，“ Comune”花粉管的形态发生了变化，并阻止了上层样式的生长，而在“ Monreal”中，它们直接向着卵巢生长。此外，为了鉴定可能参与花粉-雌蕊相互作用和自交不亲和的基因，基于互补DNA扩增片段长度多态性技术进行了研究，比较了两个品种的未授粉和自花授粉方式和柱头的转录谱。该分析确定了96种单基因，例如受体样激酶，应激诱导基因，参与苯丙烷途径的转录本，转录因子以及与钙和激素信号有关的基因。令人惊讶的是，在单基因中鉴定出高百分比的活性长末端重复序列（LTR）和非LTR逆转座子，表明它们响应授粉而被激活，并且在调节自身不相容性基因中可能发挥作用。所选基因标签的定量逆转录-聚合酶链反应分析显示，在花粉萌发和花粉管伸长过程中，两种基因型之间的转录差异。

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