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首页> 外文期刊>Plant Cell Reports >Ethylene and shoot regeneration: hookless1 modulates de novo shoot organogenesis in Arabidopsis thaliana
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Ethylene and shoot regeneration: hookless1 modulates de novo shoot organogenesis in Arabidopsis thaliana

机译：乙烯和芽再生：无钩1调节拟南芥中新芽的器官发生

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摘要

We have investigated the role of ethylene in shoot regeneration from cotyledon explants of Arabidopsis thaliana. We examined the ethylene sensitivity of five ecotypes representing both poor and prolific shoot regenerators and identified Dijon-G, a poor regenerator, as an ecotype with dramatically enhanced ethylene sensitivity. However, inhibiting ethylene action with silver nitrate generally reduced shoot organogenesis in ecotypes capable of regeneration. In ecotype Col-0, we found that ethylene-insensitive mutants (etr1-1, ein2-1, ein4, ein7) exhibited reduced shoot regeneration rates, whereas constitutive ethylene response mutants (ctr1-1, ctr1-12) increased the proportion of explants producing shoots. Our experiments with ethylene over-production mutants (eto1, eto2 and eto3) indicate that the ethylene biosynthesis inhibitor gene, ETO1, can act as an inhibitor of shoot regeneration. Pharmacological elevation of ethylene levels was also found to significantly increase the proportion of explants regenerating shoots. We determined that the hookless1 (hls1-1) mutant, a suppressor of the ethylene response phenotypes of ctr1 and eto1 mutants, is capable of dramatically enhancing shoot organogenesis. The effects of ACC and loss of HLS1 function on shoot organogenesis were found to be largely additive.
机译：我们已经研究了乙烯在拟南芥子叶外植体芽再生中的作用。我们检查了代表差和多产枝条再生器的五种生态型的乙烯敏感性，并将差的再生器Dijon-G确定为乙烯敏感性大大提高的生态型。然而，用硝酸银抑制乙烯的作用通常会降低能够再生的生态型芽的器官发生。在生态型Col-0中，我们发现对乙烯不敏感的突变体（etr1-1，ein2-1，ein4，ein7）的芽再生速率降低，而组成型乙烯响应突变体（ctr1-1，ctr1-12）则增加了外植体产生芽。我们对乙烯过量生产突变体（eto1，eto2和eto3）的实验表明，乙烯生物合成抑制剂基因ETO1可以作为芽再生的抑制剂。还发现乙烯水平的药理学升高显着增加了外植体再生芽的比例。我们确定，无钩（hls1-1）突变体，ctr1和eto1突变体的乙烯反应表型的抑制剂，能够显着增强芽器官发生。发现ACC和HLS1功能丧失对芽器官发生的影响在很大程度上是累加的。

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