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首页> 外文期刊>Plant Cell Reports >Kalanchoe blossfeldiana plants expressing the Arabidopsis etr1-1 allele show reduced ethylene sensitivity
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Kalanchoe blossfeldiana plants expressing the Arabidopsis etr1-1 allele show reduced ethylene sensitivity

机译：表达拟南芥etr1-1等位基因的Kalanchoe blossfeldiana植物显示乙烯敏感性降低

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摘要

Transgenic Kalanchoe blossfeldiana Poelln. with reduced ethylene sensitivity in flowers was obtained by Agrobacterium tumefaciens-mediated transformation using the plasmid pBEO210 containing the mutant ethylene receptor gene etr1-1 from Arabidopsis thaliana under the control of the flower-specific fbp1-promoter from Petunia. Three ethylene-resistent T0 lines, 300, 324 and 331, were selected and analyzed for postharvest-performance and morphological characteristics. Line 324 was found to be infertile and only slightly less ethylene-sensitive than control-plants, but lines 300 and 331 had significantly increased ethylene-resistance and were fertile. These two lines were analyzed for copy-number of the etr1-1 gene by Southern blotting and were crossed with the ethylene-sensitive cultivar ‘Celine’ to create T1 progeny. Line 300 contains two T-DNA copies per nucleus, one of which is rearranged, and these are unlinked according to segregation data from the crossing to ‘Celine’ and PCR-analysis of progeny plants. For control plants all flowers were closed after 2 days at 2 μl l−1 ethylene, but for line 300 only 33% were closed after 10 days. Line 331 contains three T-DNA copies per nucleus and is more sensitive to ethylene than line 300. In the line 300 the etr1-1 gene was found by RT-PCR to be expressed in petals and stamens but not in carpels and sepals. Both lines 300 and 331, and their progeny, appear morphologically and physiologically identical to control plants except for the higher ethylene resistance. Line 300 and its progeny with only one T-DNA copy have very low ethylene sensitivity and may be useful in future breeding.
机译：转基因Kalanchoe blossfeldiana Poelln。在根瘤农杆菌介导的花特异性fbp1-启动子的控制下，使用含有拟南芥突变乙烯受体基因etr1-1的pBEO210质粒，通过根癌土壤杆菌介导的转化获得了对花中乙烯敏感性降低的植物。选择了三个抗乙烯的T0系300、324和331，并进行了收获后性能和形态特征的分析。发现线324是不育的，并且对乙烯的敏感性仅比对照植物低，但是线300和331具有显着提高的耐乙烯性并且是可育的。通过Southern印迹分析了这两个系的etr1-1基因的拷贝数，并与乙烯敏感的品种“ Celine”杂交以产生T1后代。品系300每个核包含两个T-DNA拷贝，其中一个被重新排列，并且根据从杂交到“ Celine”的分离数据以及后代植物的PCR分析，这些拷贝是未链接的。对于对照植物，在2天后以2μl-1乙烯关闭所有花，但是对于第300行，在10天后仅关闭33％。品系331每个核含三个T-DNA拷贝，比品系300对乙烯更敏感。在品系300中，通过RT-PCR发现etr1-1基因在花瓣和雄蕊中表达，而在心皮和萼片中不表达。品系300和331以及它们的后代在形态和生理上都与对照植物相同，除了较高的乙烯抗性。仅具有一个T-DNA拷贝的品系300及其后代具有非常低的乙烯敏感性，并且可能在将来的育种中有用。

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  • 来源
    《Plant Cell Reports》 |2008年第4期|729-737|共9页
  • 作者

    Mohsen Sanikhani; Heiko Mibus; Bjarne M. Stummann; Margrethe Serek;

  • 作者单位

    Department of Agricultural Sciences Crop Science Faculty of Life Sciences University of Copenhagen Højbakkegaard Allé 21 2630 Taastrup Denmark;

    Faculty of Natural Sciences Institute for Ornamental and Woody Plant Science Leibniz University of Hannover Herrenhäuser Str. 2 30419 Hannover Germany;

    Department of Agricultural Sciences Crop Science Faculty of Life Sciences University of Copenhagen Højbakkegaard Allé 21 2630 Taastrup Denmark;

    Faculty of Natural Sciences Institute for Ornamental and Woody Plant Science Leibniz University of Hannover Herrenhäuser Str. 2 30419 Hannover Germany;

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  • 关键词

    Agrobacterium n tumefaciens; etr1-1 gene; Flower longevity; Flower-specific fbp1-promoter; Kalanchoe blossfeldiana; Plasmid pBEO210;

    机译：根癌土壤杆菌;etr1-1基因;花寿命;花特异性fbp1-启动子;长寿花;质粒pBEO210;

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