...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell Reports >Induction of in vitro flowering in Dendrobium Madame Thong-In (Orchidaceae) seedlings is associated with increase in endogenous N6-(Δ2-isopentenyl)-adenine (iP) and N6-(Δ2-isopentenyl)-adenosine (iPA) levels
【24h】

Induction of in vitro flowering in Dendrobium Madame Thong-In (Orchidaceae) seedlings is associated with increase in endogenous N6-(Δ2-isopentenyl)-adenine (iP) and N6-(Δ2-isopentenyl)-adenosine (iPA) levels

机译：铁皮石Mad夫人在兰花中的离体开花的诱导与内源性N6 -（Δ2-异戊烯基）-腺嘌呤（iP）和N6 -（Δ2 -异戊烯基）-腺苷（iPA）水平

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We analysed the endogenous cytokinin levels of Dendrobium Madame Thong-In seedlings grown in vitro during vegetative and flowering-inductive periods. HPLC was used to fractionate the extracts and radioimmunoassay (RIA) was used for assay of zeatin (Z), dihydrozeatin (DZ), N6-(Δ2-isopentenyl)-adenine (iP) and their derivatives. Coconut water used in experiments was found to contain high level (>136 pmol ml?1) of zeatin riboside (ZR). Protocorms and seedlings cultured in medium with coconut water were found to contain 0.5–3.9 pmol g?1 FW of the cytokinins analysed. Seedlings (1.0–1.5 cm) cultured in flowering-inductive liquid medium containing 6-benzyladenine (BA, 4.4 μM) and coconut water (CW, 15%) contained up to 200 and 133 pmol g?1 FW of iP and iPA, respectively. These levels were significantly higher than all other cytokinins analysed in seedlings of the same stage and were about 80- to 150-folds higher than seedlings cultured in non-inductive medium. During the transitional (vegetative to reproductive) stage, the endogenous levels of iP (178 pmol g?1 FW) and iPA (63 pmol g?1 FW) were also significantly higher than cytokinins in the zeatine (Z) and dihydrozeatin (DZ) families in the same seedlings. Seedlings that grew on inductive medium but remained vegetative contained lower levels of iPA. The importance of the profiles of iP and its derivatives in induction of in vitro flowering of D. Madame Thong-In is discussed.
机译：我们分析了在营养和开花诱导时期体外生长的石end夫人丁字裤幼苗的内源性细胞分裂素水平。 HPLC分离提取物，放射免疫法（RIA）测定玉米蛋白（Z），二氢玉米蛋白（DZ），N6 -（Δ2 136 pmol ml？1 ）的玉米素核糖苷（ZR）。发现用椰子水在培养基中培养的原球茎和幼苗含有0.5–3.9 pmol g？1 分析的细胞分裂素的FW。在含有6-苄腺嘌呤（BA，4.4μM）和椰子水（CW，15％）的开花诱导液体培养基中培养的幼苗（1.0–1.5 cm）所含的iP高达200和133 pmol g？1 FW和iPA分别。这些水平显着高于在同一时期的幼苗中分析的所有其他细胞分裂素，并且比在非诱导培养基中培养的幼苗高约80至150倍。在过渡期（从营养到生殖），iP（178 pmol g？1 FW）和iPA（63 pmol g？1 FW）的内源性水平也显着高于zeeatine中的细胞分裂素（Z）和二氢玉米素（DZ）家族在同一幼苗中。在感应培养基上生长但仍保持营养的幼苗中iPA的含量较低。讨论了iP及其衍生物的概况在诱导丁通夫人体外开花中的重要性。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号