...
首页> 外文期刊>Plant Cell Reports >Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria)
【24h】

Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria)

机译:小孢子胚胎发生和牛cock(Saponaria vaccaria)双单倍体协议的发展

获取原文
获取原文并翻译 | 示例
           

摘要

Factors affecting microspore embryogenesis of cow cockle (Saponaria vaccaria) were evaluated including donor plant growing conditions, genotype, bud size, density, medium composition, and culture conditions. Of the two donor plant (dayight) temperature regimes evaluated (10/5°C and 20/15°C), plants grown at 20/15°C were the most embryogenic. An embryogenic frequency of greater than 350 embryos/100 buds was observed in the most embryogenic genotype, cv. ‘White Beauty’. Buds from 3–9 mm in length were evaluated for their embryogenic potential; buds that were 4–7.9 mm produced the most embryos/100 buds. Of all the media compositions evaluated, NLN medium with 15% sucrose resulted in the most embryos. Cow cockle microspores required an initial period of 32°C for 3 days for production of microspore-derived embryos (MDEs).
机译:评价了影响牛蛤小孢子胚胎发生的因素,包括供体植物的生长条件,基因型,芽大小,密度,培养基组成和培养条件。在评估的两种供体植物(昼/夜)温度模式中(10/5°C和20/15°C),在20/15°C下生长的植物成胚性最高。在大多数胚胎发生基因型cv中,观察到大于350个胚胎/ 100个芽的胚胎发生频率。 “白色美女”。评估3–9毫米长的芽的胚发生潜能。 4–7.9毫米的芽产生最多的胚/ 100个芽。在所有评估的培养基组合物中,含15%蔗糖的NLN培养基产生最多的胚胎。牛蛤小孢子需要32°C的初始时间,持续3天才能生产小孢子衍生的胚胎(MDE)。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号