High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts

Yinü Li; Meng Sun; Jixing Liu; Zongqi Yang; Zhifang Zhang; Guifang Shen

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High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts

机译：口蹄疫病毒结构蛋白VP1在烟草叶绿体中的高表达

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A tobacco chloroplast expression vector, pTRVP1, containing the foot-and-mouth disease virus (FMDV) VP1 gene and the selective marker aadA gene, was constructed and transferred to tobacco by biolistic method. Three resistant lines were obtained through spectinomycin selection, and each transgenic line was subjected to a second round of spectinomycin selection. PCR and PCR southern blot analysis revealed that the VP1 gene had integrated into the chloroplast genome. Western blot and quantification ELISA assays indicated that the VP1 gene was expressed in tobacco chloroplasts and accounted for 2–3% of total soluble protein. This suggested that plant chloroplasts were an efficient expression system for the potential production of recombinant antigens in plants.

机译：构建了包含口蹄疫病毒（FMDV）VP1基因和选择标记aadA基因的烟草叶绿体表达载体pTRVP1，并通过基因枪法将其转移到烟草中。通过壮观霉素的选择获得了三个抗性品系，并对每个转基因品系进行了第二轮壮观霉素的选择。 PCR和PCR Southern印迹分析表明VP1基因已整合到叶绿体基因组中。 Western印迹和定量ELISA分析表明，VP1基因在烟草叶绿体中表达，占可溶性蛋白总量的2-3％。这表明植物叶绿体是在植物中潜在产生重组抗原的有效表达系统。

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来源
《Plant Cell Reports》 |2006年第4期|329-333|共5页
作者
Yinü Li; Meng Sun; Jixing Liu; Zongqi Yang; Zhifang Zhang; Guifang Shen;
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作者单位

Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China;

Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China;

Lan Zhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 P.R. China;

Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China;

Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China;

Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China;

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原文格式 PDF
正文语种 eng
中图分类
关键词
Foot-and-mouth disease virus (FMDV); VP1 protein; Tobacco transformation; Chloroplast;

机译：口蹄疫病毒;VP1蛋白;烟草转化;叶绿体;

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专利

1. High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts [J] . Li Y, Sun M, Liu J, Plant Cell Reports . 2006,第4期

机译：口蹄疫病毒结构蛋白VP1在烟草叶绿体中的高表达
2. Expression, Purification and Activity Detection of Structural Protein VP1 of Foot-and-Mouth Disease Virus Serotype A [J] . LU Qing-xia, LIU Chang, XING Guang-xu, 动物与饲料科学：英文版 . 2013,第005期

机译：甲型口蹄疫病毒结构蛋白VP1的表达，纯化及活性检测
3. Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast [J] . Sun M., Qian KX., Su N., Biotechnology Letters . 2003,第13期

机译：口蹄疫病毒VP1蛋白与莱茵衣藻叶绿体中表达的霍乱毒素B亚基融合
4. Immunization of DNA vaccine encoding C3d-VP1 fusion enhanced protective immune response against foot-and-mouth disease virus [C] . Huiying FAN, Tiezhu TONG, Huanchun CHEN, Proceedings of the 3rd Congress of the Asian Pig Veterinary Society . 2007

机译：编码C3d-VP1融合蛋白的DNA疫苗的免疫增强了针对口蹄疫病毒的保护性免疫应答
5. Expression and structural investigations of the vaccinia virus protein VP11. [D] . Singh, Adrian. 2009

机译：痘苗病毒蛋白VP11的表达和结构研究。
6. Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins [O] . Xinsheng Liu, Jianliang Lv, Yuzhen Fang, 2006

机译：包含口蹄疫病毒结构蛋白VP1和DC-SIGN结合糖蛋白的两种重组融合蛋白的表达和免疫原性
7. Influence of plant-derived protease inhibitors on the expression of the VP1 antigen from foot-and-mouth disease virus in tobacco [O] . Pillay P., Vorster J., Kunert K.J., 2010

机译：植物源蛋白酶抑制剂对口蹄疫病毒烟草中VP1抗原表达的影响

High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts

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