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首页> 外文期刊>Plant Cell Reports >Targeted overexpression of the Escherichia coli MinC protein in higher plants results in abnormal chloroplasts
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Targeted overexpression of the Escherichia coli MinC protein in higher plants results in abnormal chloroplasts

机译：在高等植物中有针对性地表达大肠杆菌MinC蛋白导致叶绿体异常

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摘要

Higher plant chloroplast division involves some of the same types of proteins that are required in prokaryotic cell division. These include two of the three Min proteins, MinD and MinE, encoded by the min operon in bacteria. Noticeably absent from annotated sequences from higher plants is a MinC homologue. A higher plant functional MinC homologue that would interfere with FtsZ polymerization, has yet to be identified. We sought to determine whether expression of the bacterial MinC in higher plants could affect chloroplast division. The Escherichia coli minC (EcMinC) gene was isolated and inserted behind the Arabidopsis thaliana RbcS transit peptide sequence for chloroplast targeting. This TP-EcMinC gene driven by the CaMV 35S2 constitutive promoter was then transformed into tobacco (Nicotiana tabacum L.). Abnormally large chloroplasts were observed in the transgenic plants suggesting that overexpression of the E. coli MinC perturbed higher plant chloroplast division.
机译：高等植物叶绿体分裂涉及原核细胞分裂所需的某些相同类型的蛋白质。这些包括由细菌中的最小操纵子编码的三个Min蛋白中的两个MinD和MinE。 MinC同源物显然不在高等植物的注释序列中。尚未发现会干扰FtsZ聚合的更高植物功能性MinC同源物。我们试图确定高等植物中细菌MinC的表达是否会影响叶绿体分裂。分离大肠杆菌minC（EcMinC）基因，并将其插入拟南芥RbcS转运肽序列的后面，用于叶绿体靶向。然后，将由CaMV 35S2组成型启动子驱动的TP-EcMinC基因转化为烟草（Nicotiana tabacum L.）。在转基因植物中观察到异常大的叶绿体，表明大肠杆菌MinC的过表达干扰了较高的植物叶绿体分裂。

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