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首页> 外文期刊>Plant Cell Reports >TDZ, auxin and genotype effects on leaf organogenesis in Fragaria
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TDZ, auxin and genotype effects on leaf organogenesis in Fragaria

机译：TDZ，生长素和基因型对草莓叶片器官发生的影响

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摘要

The different types of organogenic (roots and adventitious shoots) and callus formation responses of leaves from 30-day-old proliferating shoots of different Fragaria spp. genotypes were studied in response to MS medium supplemented with 4.54 μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (thidiazuron; TDZ) alone and in combination with 0.98 μM indole-3-butyric acid (IBA), 0.84 μM3-benzo[b]selenienyl acetic acid (BSAA) or 0.90 μM2,4-dichlorophenoxy acetic acid (2,4-D). The study included: nine octoploid Fragaria x ananassa cultivars and breeding selections; two octoploid breeding selections from F. virginiana glauca inter-species crosses; two diploid F. vesca cultivars; and one diploid clone of F. nubicola Lindl. TDZ plus IBA promoted the highest shoot regeneration efficiencies from leaves of nearly all of the genotypes, while the TDZ/BSAA and TDZ/2,4-D combinations promoted high regeneration efficiencies for only some of the genotypes (Alpina W.O., Sveva, AN 91.371.53, Onda, Paros and FO93.143.5). For the more efficient regenerating genotypes, IBA induced the highest frequency of regenerating leaves, while BSAA induced the highest number of regenerated shoots from leaves and more callus production for most of the genotypes.
机译：来自不同草莓属（Fragaria spp）的30天龄增殖芽的叶片的不同类型的器官发生（根和不定芽）和愈伤组织形成响应。对单独添加4.54μM1-苯基-3-（1,2,3-噻二唑-5-基）尿素（噻二唑; TDZ）并与0.98μM吲哚-3-丁酸联合使用的MS培养基的响应对基因型进行了研究（IBA），0.84μM3-苯并[b]硒烯基乙酸（BSAA）或0.90μM2,4-二氯苯氧基乙酸（2,4-D）。该研究包括：九种八倍体草莓属（Fragaria x ananassa）品种和育种选择。弗吉尼亚种间种间杂交的两种八倍体育种选择；两个二倍体vesca品种;和一个F. nubicola Lindl的二倍体克隆。 TDZ和IBA促进了几乎所有基因型叶片的最高嫩芽再生效率，而TDZ / BSAA和TDZ / 2,4-D组合仅促进了某些基因型的高再生效率（Alpina WO，Sveva，AN 91.371 .53，Onda，Paros和FO93.143.5）。对于更有效的再生基因型，IBA诱导了叶片再生的最高频率，而BSAA诱导了大多数基因型的叶片再生枝的最高数量和愈伤组织的产生。

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  • 来源
    《Plant Cell Reports》 |2006年第4期|281-288|共8页
  • 作者

    L. Landi; B. Mezzetti;

  • 作者单位

    Dipartimento di Scienze Ambientali e delle Produzioni Vegetali Marche Polytechnic University Via Brecce Bianche 60100 Ancona Italy;

    Dipartimento di Scienze Ambientali e delle Produzioni Vegetali Marche Polytechnic University Via Brecce Bianche 60100 Ancona Italy;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Strawberry; Leaf tissue; TDZ; BSAA; Shoot regeneration;

    机译：草莓;叶片组织;TDZ;BSAA;射出再生;

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