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首页> 外文期刊>Plant Cell Reports >A model wheat cultivar for transformation to improve resistance to Fusarium Head Blight
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A model wheat cultivar for transformation to improve resistance to Fusarium Head Blight

机译：一种用于转化以提高对枯萎病的抗性的模型小麦品种

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摘要

Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening for FHB resistance on flowers at anthesis, the number of screens carried out in a year is limited. Our objective was to evaluate the feasibility of using the rapid-maturing dwarf wheat cultivar Apogee as an alternative genotype for transgenic FHB resistance research. Our transformation efficiency (number of transgenic plantsumber of embryos) for Apogee was 1.33%. Apogee was also found to exhibit high FHB susceptibility and reached anthesis within 4 weeks. Interestingly, microsatellite marker haplotype analysis of the chromosome 3BS FHB resistant quantitative trait locus (QTL) region indicated that this region maybe deleted in Apogee. Our results indicate that Apogee is particularly well suited for accelerating transgenic FHB resistance research and transgenic wheat research in general.
机译：镰刀镰刀菌主要由禾谷镰刀菌引起，是小麦（Triticum aestivum）的主要病害。基因工程具有增强小麦对FHB抗性的巨大潜力。由于需要对花期的FHB抗性进行筛选，因此一年进行的筛选次数受到限制。我们的目标是评估使用速熟矮矮小麦品种Apogee作为转基因FHB抗性研究的替代基因型的可行性。我们对Apogee的转化效率（转基因植物数/胚数）为1.33％。还发现Apogee具有很高的FHB敏感性，并在4周内达到了花期。有趣的是，对染色体3BS FHB抗性定量性状基因座（QTL）区域的微卫星标记单倍型分析表明，该区域可能在Apogee中缺失。我们的结果表明，Apogee特别适合总体上加快转基因FHB抗性研究和转基因小麦研究。

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  • 来源
    《Plant Cell Reports》 |2006年第4期|313-319|共7页
  • 作者

    Caroline A. Mackintosh; David F. Garvin; Lorien E. Radmer; Shane J. Heinen; Gary J. Muehlbauer;

  • 作者单位

    USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics University of Minnesota 411 Borlaug Hall 1991 Upper Buford Circle St Paul MN 55108 USA;

    USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics University of Minnesota 411 Borlaug Hall 1991 Upper Buford Circle St Paul MN 55108 USA;

    USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics University of Minnesota 411 Borlaug Hall 1991 Upper Buford Circle St Paul MN 55108 USA;

    USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics University of Minnesota 411 Borlaug Hall 1991 Upper Buford Circle St Paul MN 55108 USA;

    USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics University of Minnesota 411 Borlaug Hall 1991 Upper Buford Circle St Paul MN 55108 USA;

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  • 正文语种 eng
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  • 关键词

    Apogee; Fusarium graminearum; Fusarium head blight; Transgenic wheat; Triticum aestivum;

    机译：apogee;禾谷镰刀菌;枯萎病镰刀菌;转基因小麦;普通小麦;

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