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首页> 外文期刊>Plant Cell >The indeterminate gametophyte1 Gene of Maize Encodes a LOB Domain Protein Required for Embryo Sac and Leaf Development
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The indeterminate gametophyte1 Gene of Maize Encodes a LOB Domain Protein Required for Embryo Sac and Leaf Development

机译:玉米的不确定的gametophyte1基因编码胚囊和叶片发育所需的LOB域蛋白

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摘要

Angiosperm embryo sac development begins with a phase of free nuclear division followed by cellularization and differentiationnof cell types. The indeterminate gametophyte1 (ig1) gene of maize (Zea mays) restricts the proliferative phase ofnfemale gametophyte development. ig1 mutant female gametophytes have a prolonged phase of free nuclear divisionsnleading to a variety of embryo sac abnormalities, including extra egg cells, extra polar nuclei, and extra synergids. Positionalncloning of ig1 was performed based on the genome sequence of the orthologous region in rice. ig1 encodes a LATERALnORGAN BOUNDARIES domain protein with high similarity to ASYMMETRIC LEAVES2 of Arabidopsis thaliana. A secondnmutant allele of ig1 was identified in a noncomplementation screen using active Mutator transposable element lines.nHomozygous ig1 mutants have abnormal leaf morphology as well as abnormal embryo sac development. Affected leavesnhave disrupted abaxial–adaxial polarity and fail to repress the expression of meristem-specific knotted-like homeoboxn(knox) genes in leaf primordia, causing a proliferative, stem cell identity to persist in these cells. Despite the superficialnsimilarity of ig1-O leaves and embryo sacs, ectopic knox gene expression cannot be detected in ig1-O embryo sacs.
机译:被子植物胚囊的发育开始于一个自由核分裂阶段,随后是细胞类型的细胞化和分化。玉米(Zea mays)的不确定配子体1(ig1)基因限制了女性配子体发育的增殖期。 ig1突变的雌配子体具有延长的自由核分裂期,导致多种胚囊异常,包括额外的卵细胞,额外的极核和额外的协同作用。基于水稻直系同源区的基因组序列对ig1进行定位。 ig1编码与拟南芥的ASYMMETRIC LEAVES2具有高度相似性的LATERALnORGAN BOUNDARIES域蛋白。 ig1的第二个突变体等位基因在非互补筛选中使用活跃的Mutator转座元件系进行鉴定。n纯合的ig1突变体具有异常的叶片形态以及异常的胚囊发育。受影响的叶子已经破坏了叶的正反极性,并且不能抑制叶原基中分生组织特异的打结状同源异型素(knox)基因的表达,从而导致增殖的干细胞特性在这些细胞中持续存在。尽管ig1-O叶片和胚囊表面相似,但在ig1-O胚囊中无法检测到异位诺克斯基因表达。

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  • 来源
    《Plant Cell》 |2007年第1期|p.46-62|共17页
  • 作者

    Matthew M.S. Evans;

  • 作者单位

    Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    indeterminate gametophyte1 (ig1);

    机译:不确定配子体1(ig1);

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