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A simple colorimetric method for determination of hydrogen peroxide in plant tissues

机译：一种简单的比色法测定植物组织中的过氧化氢

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摘要

A simple colorimetric method for determination of hydrogen peroxide in plant materials is described. The method is based on hydrogen peroxide producing a stable red product in reaction with 4-aminoantipyrine and phenol in the presence of peroxidase. Plant tissues was ground with trichloroacetic acid (5% w/v) and extracts were adjusted to pH 8.4 with ammonia solution. Activated charcoal was added to the homogenate to remove pigments, antioxidants and other interfering substances. The colorimetric reagent (pH 5.6) consisted of 4-aminoantipyrine, phenol, and peroxidase. With this method, we have determined the hydrogen peroxide concentration in leaves of eight species which ranged from 0.2 to 0.8 µmol g−1 FW. Changes in hydrogen peroxide concentration of Stylosanthes guianensis in response to heat stress are also analyzed using this method.
机译：描述了一种用于测定植物材料中过氧化氢的简单比色法。该方法基于过氧化氢在过氧化物酶存在下与4-氨基安替比林和苯酚反应生成稳定的红色产物。用三氯乙酸（5％w / v）研磨植物组织，并用氨溶液将提取物的pH调节至8.4。将活性炭加入匀浆中，以除去色素，抗氧化剂和其他干扰物质。比色试剂（pH 5.6）由4-氨基安替比林，苯酚和过氧化物酶组成。通过这种方法，我们确定了八种物种的叶片中过氧化氢的浓度，其范围为0.2至0.8 µmol g-1 FW。还使用此方法分析了响应热应激的圭花香过氧化氢浓度的变化。

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  • 来源
    《Plant Growth Regulation》 |2006年第3期|113-118|共6页
  • 作者

    Biyan Zhou; Jihua Wang; Zhenfei Guo; Huoquan Tan; Xiaochuan Zhu;

  • 作者单位

    College of Life Science South China Agricultural UniversityPhysiological Laboratory for South China Fruits College of Horticulture South China Agricultural University;

    College of Life Science South China Agricultural University;

    College of Life Science South China Agricultural University;

    College of Life Science South China Agricultural University;

    Physiological Laboratory for South China Fruits College of Horticulture South China Agricultural University;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    4-aminoantipyrine; Hydrogen peroxide; Peroxidase; Plant tissue;

    机译：4-氨基安替比林;过氧化氢;过氧化物酶;植物组织;

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