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首页> 外文期刊>Plant Growth Regulation >Isolation of cDNA clones for genes that are differentially expressed during thermoinhibition in achenes of Tagetes minuta L.
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Isolation of cDNA clones for genes that are differentially expressed during thermoinhibition in achenes of Tagetes minuta L.

机译：分离塔吉格氏瘦果瘦果热抑制过程中差异表达基因的cDNA克隆。

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摘要

Differential display was used to isolate genes which are expressed specifically in thermoinhibited achenes of Tagetes minuta L. Of the 62 thermoinhibition-associated cDNAs identified, thirteen were cloned. Three clones for each cDNA were selected and reverse northern analysis used to confirm that all 39 clones were specific to the mRNA pool of thermoinhibited achenes only. High quality sequence data were obtained for 27 of these. Alignment of the various sequences revealed that a total of 14 different sequences had been cloned, indicating that several of the bands isolated from the differential display gels contained multiple sequences. Electronic homology searches tentatively identified three of the sequences, whilst the remainder did not show any significant homology to known sequences. Of the sequences identified, one may encode a plant transcription factor-like or nuclear RNA-binding protein, another an RNase-L inhibitor-like protein and the third a miraculin homologue. The potential roles of these genes in the imposition and maintenance of the thermoinhibited state are discussed. These results are consistent with the hypothesis that thermoinhibition in T. minuta achenes is under positive genetic control, in a manner analogous to embryo dormancy.
机译：使用差异展示法分离了在热抑制的万寿菊中的瘦果中特异性表达的基因。在鉴定的62个与热抑制相关的cDNA中，克隆了13个。为每个cDNA选择三个克隆，并进行反向Northern分析以确认所有39个克隆仅对热抑制瘦果的mRNA库具有特异性。其中27个获得了高质量的序列数据。各种序列的比对揭示总共克隆了14个不同的序列，表明从差异展示凝胶分离的几个条带包含多个序列。电子同源性搜索初步确定了三个序列，而其余序列与已知序列没有任何显着同源性。在所鉴定的序列中，一个可以编码植物转录因子样或核RNA结合蛋白，另一个可以编码RNase-L抑制剂样蛋白，而第三个可以编码奇迹蛋白同系物。讨论了这些基因在热抑制状态的施加和维持中的潜在作用。这些结果与假单胞菌瘦果中的热抑制以类似于胚胎休眠的方式在积极的遗传控制下的假设相一致。

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