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Characterizing Polygala L. (Polygalaceae) Species in Southern Brazil Using ISSR

机译：使用ISSR表征巴西南部远志（Polygalaceae）物种

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The genus Polygala is one of the seven Polygalaceae genera that occur in the Brazilian flora, covering approximately 110 species. During the taxonomic review of Polygalaceae in Southern Brazil, difficulties were found when classifying species with very similar morphology, and morphological data alone could not clarify these interspecific relationships. In this context, inter-simple sequence repeat (ISSR) molecular markers were used in an attempt to characterize the genetic diversity and relationships among Polygala species. Nine Polygala species were analyzed using six selected ISSR primers that generated a total of 75 bands (100% polymorphic). The relationships were evaluated by dendrograms using the unweighted pair group method using arithmetic averages algorithm. The use of ISSR to solve the taxonomic problems was very useful for the Brazilian Polygala species. This is the first report of a molecular characterization of the Brazilian Polygala species to successfully group the different species. The ISSR results are in agreement with the morphological evidence of a new Polygala species from Southern Brazil.
机译：远志属是巴西植物区系中的七个远志科之一，覆盖约110种。在巴西南部远志科的分类学研究中，当对形态非常相似的物种进行分类时发现困难，仅形态学数据无法阐明这些种间关系。在这种情况下，使用简单序列间重复（ISSR）分子标记来表征远志物种之间的遗传多样性和关系。使用六种选定的ISSR引物分析了九种远志物种，这些引物共产生75条带（100％多态）。通过使用算术平均值算法的非加权对组方法，通过树状图评估这些关系。利用ISSR解决分类问题对于巴西Polygala物种非常有用。这是首次报道巴西远志物种成功地将不同物种归类的分子报告。 ISSR结果与来自巴西南部的一种新远志物种的形态学证据相符。

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