...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Molecular Biology >Vernalization Response in Perennial Ryegrass (Lolium perenne L.) Involves Orthologues of Diploid Wheat (Triticum monococcum) VRN1 and Rice (Oryza sativa) Hd1
【24h】

Vernalization Response in Perennial Ryegrass (Lolium perenne L.) Involves Orthologues of Diploid Wheat (Triticum monococcum) VRN1 and Rice (Oryza sativa) Hd1

机译：多年生黑麦草（Lolium perenne L.）的春化反应涉及二倍体小麦（Triticum monococcum）VRN1和水稻（Oryza sativa）Hd1的直向同源物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Flowering time is important when adapting crop plants to different environments. While high feeding quality of forage grasses is facilitated by repression of flowering, flowering should also be inducible to facilitate grass seed production. Consequently, the identification and characterization of the genes controlling flowering time in forage grasses, including perennial ryegrass (Lolium perenne L.), is of great interest. In this study, three candidate genes for vernalization response genes in perennial ryegrass were identified based on DNA sequence homology to TmVRN1 and TmVRN2 of diploid wheat (Triticum monococcum), and Hd1 of rice (Oryza sativa). High sequence similarity between LpVRN1 and TmVRN1, co-localization of LpVRN1 with a major quantitative trait loci (QTL) for vernalization response in perennial ryegrass, synteny between map-positions of LpVRN1 and TmVRN1, mRNA expression analysis of LpVRN1 alleles during vernalization, and the correspondence between LpVRN1 mRNA expression levels and flowering time leads us to conclude that LpVRN1 is orthologous to TmVRN1 and that its function is conserved between diploid wheat and perennial ryegrass. Of the remaining two candidate genes, a putative Hd1 orthologue, LpCO, co-localized with a second QTL for vernlization response. LpCO has recently been shown to be involved in the photoperiodic regulation of flowering time. While epistasis, at the level of LpVRN1 transcription, was observed between the LpVRN1 and LpCO genomic regions, no differential expression of LpCO transcripts was observed during vernalization. While orthologous genes controlling flowering time can thus be identified, future allele sequencing efforts will reveal if causative polymorphisms are conserved across the grasses.
机译：当使农作物适应不同环境时，开花时间很重要。虽然抑制开花有助于饲草的高饲喂质量，但也应诱导开花以促进草种子的生产。因此，在包括多年生黑麦草（Lolium perenne L.）在内的饲草中控制开花时间的基因的鉴定和表征引起了极大的兴趣。在这项研究中，根据与二倍体小麦（Triticum monococcum）的TmVRN1和TmVRN2和水稻（Oryza sativa）的Hd1的DNA序列同源性，确定了多年生黑麦草中春化应答基因的三个候选基因。 LpVRN1和TmVRN1之间的序列相似性高，LpVRN1与多年生黑麦草的春化反应的主要定量性状基因座（QTL）共定位，LpVRN1和TmVRN1的图谱位置之间的一致性，春化期间LpVRN1等位基因的mRNA表达分析，以及LpVRN1 mRNA表达水平和开花时间之间的对应关系使我们得出结论，LpVRN1与TmVRN1同源，并且其功能在二倍体小麦和多年生黑麦草之间是保守的。在其余的两个候选基因中，推定的Hd1直向同源物LpCO与第二个QTL共同定位，以进行vernization反应。 LpCO最近已显示出参与开花时间的光周期调节。虽然在LpVRN1和LpCO基因组区域之间观察到了LpVRN1转录水平的上位性，但在春化期间未观察到LpCO转录本的差异表达。因此，虽然可以确定控制开花时间的直系同源基因，但将来的等位基因测序工作将揭示出是否在草地上保留了致病性多态性。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号