Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-22 from Lycopersicon esculentum

Frank C. Lanfermeijer; Jos Dijkhuis; Marcel J.G. Sturre; Peter de Haan; Jacques Hille

首页> 外文期刊>Plant Molecular Biology >Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-22 from Lycopersicon esculentum

【24h】

Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-22 from Lycopersicon esculentum

机译：番茄番茄持久性抗番茄花叶病毒基因Tm-22 的克隆与鉴定

获取原文

获取原文并翻译 | 示例

获取外文期刊封面封底 >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

In tomato, infections by tomato mosaic virus are controlled by durable Tm-22 resistance. In order to gain insight into the processes underlying disease resistance and its durability, we cloned and analysed the Tm-22 resistance gene and the susceptible allele, tm-2. The Tm-22 gene was isolated by transposon tagging using a screen in which plants with a destroyed Tm-22 gene survive. The Tm-22 locus consists of a single gene that encodes an 861 amino acid polypeptide, which belongs to the CC-NBS-LRR class of resistance proteins. The putative tm-2 allele was cloned from susceptible tomato lines via PCR with primers based on the Tm-22 sequence. Interestingly, the tm-2 gene has an open reading frame that is comparable to the Tm-22 allele. Between the tm-2 and the Tm-22 polypeptide 38 amino acid differences are present of which 26 are located in the second half of the LRR-domain. Susceptible tomato plants, which were transformed with the Tm-22 gene, displayed resistance against ToMV infection. In addition, virus specificity, displayed by the Tm-22 resistance was conserved in these transgenic lines. To explain the durability of this resistance, it is proposed that the Tm-22-encoded resistance is aimed at the Achilles' heel of the virus.

机译：在番茄中，番茄花叶病毒的感染受持久的Tm-22 抗性控制。为了深入了解疾病抗性及其持久性的过程，我们克隆并分析了Tm-22 抗性基因和易感等位基因tm-2。 Tm-22 基因是通过转座子标签分离得到的，该屏幕上的Tm-22 基因被破坏。 Tm-22 基因座由一个编码861个氨基酸的多肽的单一基因组成，该多肽属于CC-NBS-LRR类抗性蛋白。通过基于Tm-22 序列的引物PCR，从易感番茄品系中克隆出推定的tm-2等位基因。有趣的是，tm-2基因的开放阅读框与Tm-22 等位基因相当。在tm-2和Tm-22 多肽之间存在38个氨基酸差异，其中26个位于LRR结构域的后半部分。用Tm-22 基因转化的易感番茄植株对ToMV感染具有抗性。此外，在这些转基因品系中，由Tm-22 抗性表现出的病毒特异性得以保留。为了解释这种抗药性的持久性，建议将Tm-22 编码的抗药针对该病毒的致命弱点。

著录项

来源
《Plant Molecular Biology》 |2003年第5期|1039-1051|共13页
作者
Frank C. Lanfermeijer; Jos Dijkhuis; Marcel J.G. Sturre; Peter de Haan; Jacques Hille;
展开▼
作者单位

Department Molecular Biology of Plants Groningen Biomolecular Sciences and Biotechnology Institute University of GroningenLaboratory of Plant Physiology Department of Plant Biology University of Groningen;

Department Molecular Biology of Plants Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen;

Department Molecular Biology of Plants Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen;

Syngenta Seeds B.V.Viruvation B.V.;

Department Molecular Biology of Plants Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词
CC-NBS-LRR; Lycopersicon esculentum; plant disease resistance gene; Tm-22; tomato mosaic virus; transposon tagging;

机译：CC-NBS-LRR;番茄;植物抗病基因;Tm-22;番茄花叶病毒;转座子标记;

相似文献

外文文献
中文文献
专利

1. Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-2(2) from Lycopersicon esculentum [J] . Lanfermeijer FC., Dijkhuis J., Sturre MJG., Plant Molecular Biology . 2003,第5期

机译：番茄番茄持久性抗番茄花叶病毒基因Tm-2（2）的克隆与鉴定
2. First report in Spain of a variant of Tomato mosaic virus (ToMV) overcoming the Tm-2~2 resistance gene in tomato (Lycopersicon esculentum) [J] . J.Aramburu, L.Galipienso Plant Pathology . 2005,第4期

机译：西班牙首次报道了克服番茄中Tm-2〜2抗性基因的番茄花叶病毒（ToMV）变种（Lycopersicon esculentum）
3. Linkage between Frl (Fusarium oxysporum f.sp. radicis-lycopersici resistance) and Tm-2 (tobacco mosaic virus resistance-2) loci in tomato (Lycopersicon esculentum). [J] . Vakalounakis DJ, Laterrot H, Moretti A, Annals of Applied Biology . 1997,第2期

机译：番茄（Lycopersicon esculentum）的Fr1（尖孢镰刀菌对萝卜-lycopersici抗性）和Tm-2（烟草花叶病毒抗性-2）基因座之间的联系。
4. Chlorophyll Fluorescence Analysis of Tomato Plants (Lycopersicon esculentum L.) Infected with Tomato Mosaic Virus (ToMV) [C] . Dora KREZHOVA, Ilko ILIEV, Tony YANEV, European Association of Remote Sensing Laboratories Symposium . 2010

机译：番茄植物（Lycopersicon Esculentum L.）感染番茄马赛克病毒（TOMV）的叶绿素荧光分析
5. Molecular cloning and characterization of the diageotropica gene in tomato (Lycopersicon esculentum Mill.). [D] . Oh, KwangChul. 2004

机译：番茄中的diageotropica基因的分子克隆和表征（Lycopersicon esculentum Mill。）。
6. Sequencing of cDNA Clones from the Genetic Map of Tomato (Lycopersicon esculentum) [O] . Martin W. Ganal, Rosemarie Czihal, Ulrich Hannappel, 1998

机译：番茄（Lycopersicon esculentum）遗传图谱中cDNA克隆的测序
7. Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-22 from Lycopersicon esculentum [O] . Lanfermeijer Frank C., Dijkhuis Jos, Sturre Marcel J.G., 2003

机译：番茄Lycopersicon esculentum的耐番茄花叶病毒抗性基因Tm-22的克隆与鉴定

Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-22 from Lycopersicon esculentum

摘要

著录项

相似文献

相关主题

期刊订阅