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首页> 外文期刊>Plant Physiology >Modifications to the Arabidopsis Defense Proteome Occur Prior to Significant Transcriptional Change in Response to Inoculation with Pseudomonas syringae
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Modifications to the Arabidopsis Defense Proteome Occur Prior to Significant Transcriptional Change in Response to Inoculation with Pseudomonas syringae

机译：拟南芥防御蛋白质组的修饰发生在对丁香假单胞菌接种产生应答的显着转录变化之前

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摘要

Alterations in the proteome of Arabidopsis (Arabidopsis thaliana) leaves during responses to challenge by Pseudomonas syringae pv tomato DC3000 were analyzed using two-dimensional gel electrophoresis. Protein changes characteristic of the establishment of disease, basal resistance, and resistance-gene-mediated resistance were examined by comparing responses to DC3000, a hrp mutant, and DC3000 expressing avrRpm1, respectively. The abundance of each protein identified was compared with that of selected transcripts obtained from comparable GeneChip experiments. We report changes in three subcellular fractions: total soluble protein, chloroplast enriched, and mitochondria enriched over four time points (1.5–6 h after inoculation). In total, 73 differential spots representing 52 unique proteins were successfully identified. Many of the changes in protein spot density occurred before significant transcriptional reprogramming was evident between treatments. The high proportion of proteins represented by more than one spot indicated that many of the changes to the proteome can be attributed to posttranscriptional modifications. Proteins found to show significant change after bacterial challenge are representative of two main functional groups: defense-related antioxidants and metabolic enzymes. Significant changes to photosystem II and to components of the mitochondrial permeability transition were also identified. Rapid communication between organelles and regulation of primary metabolism through redox-mediated signaling are supported by our data.
机译：使用二维凝胶电泳分析了拟南芥丁香假单胞菌pv番茄DC3000对攻击的应答期间拟南芥（Arabidopsis thaliana）叶片蛋白质组的变化。通过比较分别对表达avrRpm1的DC3000，hrp突变体和DC3000的应答，检查了疾病建立，基础抗性和抗性基因介导的抗性的蛋白质变化特征。将鉴定出的每种蛋白质的丰度与从类似的GeneChip实验获得的选定转录本的丰度进行比较。我们报告了三个亚细胞部分的变化：总可溶性蛋白，富集叶绿体和线粒体富集在四个时间点（接种后1.5–6小时）。总共成功鉴定出代表52种独特蛋白质的73个差异点。在处理之间明显的转录重编程之前，蛋白质斑点密度发生了许多变化。一个以上斑点代表的蛋白质比例很高，表明蛋白质组的许多变化都可以归因于转录后修饰。被发现在细菌攻击后显示出显着变化的蛋白质代表两个主要功能组：防御相关的抗氧化剂和代谢酶。还确定了光系统II和线粒体通透性转变的组成部分的重大变化。我们的数据支持细胞器之间的快速交流和通过氧化还原介导的信号调节初级代谢。

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